核转仪试剂盒说明书amaxa_OP_Basic_Mammal_Fibro_VPI-1002.pdf

page 1 of 4 ® Basic Nucleofector Protocol for Mammalian Fibroblasts DPI-1002 Vs. 08-2008 ® Basic Nucleofector Kit for Primary Mammalian Fibroblasts For Any Primary Mammalian Fibroblasts Cells derived from mammalian fibroblastoid tissues from various organs; adherent cells Note ! Mammalian fibroblasts display significant phenotypic variations due to the wide range of both species and body sites from which they may be sourced. You can determine the optimal ® ® Nucleofection condition for your fibroblasts using the Basic Nucleofector Kit for Primary Mammalian Fibroblasts [Cat. No. VPI-1002]. Please find some guidelines on fibroblast cell culture ® ® for Nucleofection and on the transfection procedure using our Basic Nucleofection Kit below. However, we recommend referring to more detailed culture protocols before you start the experiments. Having tested various fibroblastoid cell types, high transfection efficiencies could be achieved using one of the programs indicated below. In case you do not attain satisfying results with your fibroblasts of interest please contact our Scientific Support Team for further help with the optimization. Product Description