朱道弘-2007-栗瘿蜂体内Wolbachia的感染及其wsp基因序列分析_林业科学.pdfVIP

43 3 Vol.43, No.3 200 7 3 SCIENTIA SILVAE SINICAE Mar., 2 007 ＊ Wolbachia wsp 朱道弘　贺一原　赵吕权　黄向东　阳艳萍 (　410004) :　Wolbachia;wsp ;;; :S718.7　　　:A　　　:1001-7488(2007)03-0133-05 Sequencing and Phylogenetic Analysis of the wsp Gene of Wolbachia in a Gallwasp Species, Dry ocosmus kuriphilus (Hymenoptera:Cynipidae) Zhu Daohong　He iyuan　Zhao Lǜquan　Huang Xiangdong　 ang anping (College of Resource and Environment, CentralSouth University of Forestry Technology 　Changsha 410004) Abstract:　Alpha proteobacteria of the genus Wolbachia are a widespread group of maternally inherited endosymbionts of arthropod and nematode hosts.Wolbachia infection induces a range of host phenotypes, including cytoplasmic incompatibility, male killing, feminization and thelytoky induction.Heterogony (cyclical parthenogenesis)is a remarkable characteristic of oak gall wasps, Cynipini, which is the largest tribe in Cynipidae.A few species of Cynipini are exceptionally, which are univoltine and exhibit thelytokous parthenogenesis, probably due to deletion of the arrhenotokousgeneration from their heterogonic ancestor specieswith Wolbachia infection.In this study, the presence of Wolbachia was detectedusing polymerase chain reaction (PCR) primers for the wsp genes in a thelytokous species (Dryocosmus kuriphilus)of the Cynipini.The wsp characteristic 617 bp product was obtainedafter amplificationof extractsfromZhuzhoupopulation.The sequence clearly conformed to Wolbachia group A andwas nearly identical to that of lagiotrochus quercusilicis.The possible implicationsof Wolbachia infection inthe gallwasp are discussed. Key words:　Wolbachia;wsp gene;sequence analysi