NMDA受体机制研究.ppt

Literature Report Group： Nerve Protection Reporter： 胡晓龙 2013.06.15 前 言 4 CONTENT 前 言 English Literature 1 Multiple Targets in Neuroprotection 2 Part1 English Paper Neurochemistry Research IF:2.24 Section1 Introduction 1.Oxidative stress has long been implicated in the neurodegenerative diseases, includingAD,PDand ischemic injury 2.In many cells, ROS caused DNA damage, oxidative of proteins, and thus induced apoptosis.Therefore, therapeutic strategies aimed at preventing or delaying ROS production might be a reasonable choice for the treatment of these diseases. Section2 Experimental Procedure 1.Chemicals and Reagents 2. Cell Culture 3. MTT Assay 4. Hoechst 33258 Staining 5. Western Blot Assay 6.Statistical Analysis Section3 Results Dicussion Result-1 H2O2-Induced Cytotoxicity in SH-SY5Y Cells Result-2 Ugonin K Prevented SH-SY5Y Cells Against H2O2-Induced Cell Death Result-3 Ugonin K Prevents H2O2-Induced Activation of Caspase-3 Result-4 Ugonin K Mediated Protective Action Involves Extracellular Signal Regulated Kinase 1 and 2 (ERK1/2) Activation Result-5 Ugonin K Mediated Protective Action Involves PI3K- Dependent Akt Activation Result-6 JNK1/2 and P38 are not Involved in SH-SY5Y Cells Protective Against H2O2-Induced Apoptosis Section3 Conclusion Ugonin K could ameliorate H2O2-induced cell death in SH-SY5Y cells. Ugonin K protected SH-SY5Y cells from oxidative stress-induced neurotoxicity may involve ERK1/2 and PI3K/Akt pathways. Therefore, the molecular mechanisms neuroprotective effects of Ugonin K may include not only their antioxidant activities but also their interaction with cell signaling cascades leading to cell survival and cell proliferation. Part2 Multiple Targets In Neuroprotection 临床实践发现高特异性的单靶标药物的临床疗效并不尽如人意。系统生物学研究表明人体内各条信号通路组成错综复杂的信号网路，阻断其中一条途径势必会引起其他通路反馈性激活或抑制，比如在多数情况下，特别是中枢神经系统疾病如AD，PD，其病因、病理机制及疾病进展过程非常复杂，通常是多囚素的，包括许多危险因子和缺陷蛋白质彼此失去平衡的信号通路，如果药物只作用在单个靶点上，那么治疗的成功率是极低的。 AchE DS2.5.5 柔性对