芥菜16个变种的RAPD研究.pdfVIP

植 　物 　学 　报 　1998 , 40 ( 10) :915～921 Acta Botanica Sinica 芥菜 16 个变种的 RAPD 研究 ① 乔爱民 　刘佩瑛　雷建军 ( 西南农业大学园艺系　重庆　630716) ( ) 摘要 　利用 RAPD 技术对芥菜 Brassica juncea Coss. 16 个变种的遗传变异进行研究。从 60 个随机引物 中筛选出27 个有效引物 ,共扩增出 336 条 DNA 带 ,其中 275 条为多态性带 , 占8185 % ,平均每个引物扩 增的 DNA 带数为 1244 条。利用 19 个有效引物扩增的 240 条 DNA 带对芥菜 16 个变种间的亲缘关系进 行 UPGA 聚类分析 ,计算出 16 个变种间的平均遗传距离为 734 ,在此基础上建立了中国菜用芥菜 16 个 变种的 DNA 分子系统树图。该系统将芥菜的 16 个变种划归 A 、B 、C 三个组 ,其中 A 组 7 个变种 ,B 组 8 个变种 ,C 组只有 1 个变种 ,B 组又可细分为两个亚组。对芥菜遗传多样性的分子基础进行了探讨。 关键词 　芥菜 ,变种 ,RAPD ,遗传多样性 RAPD ANALYSIS OF SIXTEEN VARIETIES OF MUSTARD QIAO AiMin 　LIU PeiYing 　LEI JianJun ( Department of Horticulture , Southwest Agricultural University , Chongqing 630716) Abstract 　RAPD assessment on genetic variations of 16 mustard ( Brassica juncea Coss. ) varieties were carried out. 27 primers were screened from sixty 10 bp arbitrary primers , and a total of 336 DNA bands were amplified , among which 275 (81. 85 %) were polymorphic. The average number of DNA band amplified by each primer was 12. 44 , and the average genetic distance among 16 mus tard varieties was 7. 34. Based on UPGA cluster analysis of 240 DNA bands amplified by 19 primers , a DNA molecular dendrogram was established for 16 vegetable mustard varieties in China , which divided the 16 mustard varieties into three groups : group A , B and C. Group A included sev en varieties , group B eight and group C only one. Group B could be divided into two subgroups :B1 and B . The molecular foundation of genetic diversity of mustard was explored. 2 Key words 　Brassica juncea , Variety , RAPD , Genetic diversity ( ) ( )