抑制溴酚蓝褪色动力学光度法测定茶叶中痕量铜.pdfVIP

210 ※分析检测 2013,Voi．34,No．16 良品科学 抑制溴酚蓝褪色动力学光度法测定茶叶中痕量铜 谢建鹰，邓经营 (上饶师范学院化学化工学院，江西上饶 334001) 摘要：基于在pH3．5的HAc．NaAc缓冲溶液介质中，在抗坏血酸活化剂的存在下，痕量铜可抑制碘酸钾氧化溴酚蓝 的褪色反应，建立抑制动力学光度法测定痕量铜的新方法，研究相关的测定条件和试剂用量，考察其他离子对测定 的影响。结果表明：在优化的晟佳实验条件下，方法的测定线性范围为0～0．058wdmL，检出限为9．04×10。og／mL， 样品加标准回收率在94．3％～102．6％之间，相对标准偏差均不大于4．8％。方法简便、快速，灵敏度高。用于茶叶中 铜的测定，结果满意。 关键词：抑制动力学光度法；溴酚蓝；铜；茶叶；碘酸钾 Kinetic DeterminationofTrace inTeaBasedONInhibitedof Blue Spectrophotometric Copper FadingBromophenol XIE Jian-ying，DENGJing—ying Chemical Normal and (CollegeofChemistry Engineering，ShangraoCollege，Shangrao334001，China) new kinetic methodforthedeterminationoftrace inteawas Abstract：A inhibitoryspectrophotometric copper proposed． ThemethodwasbasedonthefactthatinHAc—NaAcbuffer amountsof caninhibitthe 3．5)，trace solution(pH copper oxidativeof blueinduced iodateinthe ofascorbicacidasanactivator． fadingbromophenol bypotassium presence conditionswere andinterferencefrom ionswas Importantexperimental optimized CO—existing investigated．Underoptimized of linear themethodwas0-0．058 thelimitofdetectionwas conditions，the range lag／mL，and 9．04×10。”g／mL．The of from tea wereinthe of94．3％一102．6％withrelativestandarddevimions recoveries average copperspikedsamples range lessthanor to method tobe andhasbeenusedtodeterminetrace