利用连锁和关联分析定位粳稻芽期及幼苗前期耐盐性QTL.doc

利用连锁和关联分析定位粳稻芽期及幼苗前期耐盐性QTL 郑洪亮　刘博文　赵宏伟　王敬国　刘化龙　孙健　邢军　邹德堂 (东北农业大学水稻研究所,哈尔滨１５００３０;通讯联系人,EＧmail:zoudt＠１６３．com) IdentificationofQTLsforSaltToleranceattheGerminationandEarlySeedling StageUsingLinkageandAssociationAnalysisinjaponicaRice ZHENGHongＧliang,LIUBoＧwen,ZHAOHongＧwei,WANGJingＧguo,LIUHuaＧlong,SUNJian,XINGJun,ZOU DeＧtang (RiceResearchInstitute,NortheastAgriculturalUniversity,Harbin１５００３０,China;Correspondingauthor,EＧmail:zoudt＠１６３． com) ZHENGHongliang,LIU,Bowen,ZHAOHongwei,etal．IdentificationofQTLsforsalttoleranceatthegermination andearlyseedlingstageusinglinkageandassociationanalysisinjaponicarice．ChinJRiceSci,２０１４,２８(４):３５８Ｇ３６６． Abstract:QTLforsalttoleranceatthegerminationandearlyseedlingstageinriceweredetectedvialinkageand associationanalyses．ABC２F２:３populationwasconstructedincluding１９０BC２F３lines,derivedfromacrossbetween Dongnong４２５(highＧyieldingricevarietywithgoodgrainquality,astherecurrentparent)andChangbai１０(salt tolerantricevariety,asthedonorparent)．QTLsweredetectedforsalttoleranceatthegerminationandearlyseedling stageincludingrelativegerminationrate(RGR),relativeseedlingheight(RSH),relativerootnumber(RRN), relativerootlength(RRL)weredetectedbyusingICIMwithageneticlinkagemapconstructedwith１３７SSRmarkers． Atotalof１１QTLswereidentified．Apanelof３４１japonicariceaccessionsfromdifferentgeographicaloriginswereused forbothwholeＧgenomeassociationandtargetedregionalassociationmapping．Theaccessionsweregenotypedwith１６０ selectedSSRmarkers,andthenassociationanalysisbetweenSSRmarkersandtraitswereperformedusingTASSEL ２?１GLMandMLMprograms．Atotalof２２significantmarkerＧtraitassociationswereidentifiedwithin１８markers, and５QTLsreportedbylinkagemappingwerevalidated,and９novelallelesattheselociwereminedfromthesetof japonicarice．Bycomparingthechromosomalpositionsofthe５QTLswiththosepreviouslyidentified,fourofthem locatedinthesameorneargenomeregions,qRRL７wasreportedforthefirsttime． Keywords:japonicarice;germinationstage;earlyseedlingstage;salttolerance;QTL;linkagemapping;association mapping