HPLC和LC-MS对唑草酮原药的检测方法比较.pdfVIP

— 48 — 杂草学报 2016 年第34 卷第4期 聂天莹，陈鸿申，林绍霞，等.HPLC和LC-MS 对唑草酮原药的检测方法比较［J］.杂草学报，2016，34（4）：48 -55. HPLC 和 LC -MS对唑草酮原药的检测方法比较 聂天莹 ，陈鸿申 ，林绍霞 ，杨鸿波1 2 1 1 （1.贵州省分析测试研究院，贵州贵阳550002；2.贵州大学生命科学学院，贵州贵阳550025） 摘要：在对唑草酮进行残留分析试验的过程中，对其浓度的分析检测方法有很多。通过HPLC 和 LC-MS 对唑草酮原药的检测比较，得到HPLC对唑草酮的LOD为0.011 mg／L，LOQ为0.036 mg／L；LC-MS对唑草酮 的LOD为0.036 ng／L，LOQ为2.04 ng／L，且HPLC和LC-MS对唑草酮原药的检测方法都具有易操作、分离特 性好的特点，但LC-MS 的检出限比HPLC低，推荐LC-MS适合用来分析唑草酮含量较低的样品，而HPLC适 合用来分析唑草酮含量较高的样品。 关键词：唑草酮；HPLC；LC-MS 中图分类号：O657 文献标志码：A 文章编号：1003 -935X（2016）04 -0048 -08 Comparison ofDetection Methods of Carfentrazone Ethyl by HPLC and LC-MS NIE Tian-ying，CHEN Hong-shen，LIN Shao-xia，YANG Hong-bo （1.Guizhou Academy ofTesting and Analysis，Guiyang550002，China； 2.College ofLife Sciences，Guizhou University，Guiyang550025，China） Abstract：There are many methods for determining the carfentrazone -ethyl concentration as part ofresidue analyses. The detection of carfentrazone -ethyl by HPLC and LC -MS was compared.The LOD and LOQ by HPLC were 0.011 mg／L and0.036 mg／L，respectively.The LODand LOQby LC-MSwere0.036 ng／Land2.04 ng／L，respec- tively.Both the HPLC and LC-MS detection methodsforcarfentrazone-ethyl are easy to operate.The characteristics ofseparation properties are good butthe detection limitofLC-MS islowerthan thatofHPLC.LC-MS is suitable for the analysis of samples containing low concentrations ofcarfentrazone -ethyl and HPLC for those having high content samples ofthe herbicide. Key words：carfentrazone-ethyl；LC-MS；HPLC 唑酮草酯属原卟啉原氧化酶抑制剂，即通过 起细胞膜破坏，使叶片迅速干枯、死亡，适用于小 抑制叶绿素生物合成过程中原卟啉原氧化酶而引 麦、大麦、燕麦、水稻、玉米、大豆、柑橘、咖啡、棉