白藜芦醇对6周耐力训练小鼠红细胞形态、抗氧化和免疫功能的影响.pdfVIP

文章编号：1000—677X(2007)12—0053 04 ② 体育科学 2007年(第27卷)第12期 CHINA SP()RT SCIENCE Vo1．27，No．12，53—56，2007 白藜芦醇对6周耐力训练小鼠红细胞 形态、抗氧化和免疫功能的影响 Effects of Resveratrol on Morphology，Anti—oxidation and Immunity Capacity in Erythrocyte of Mice Performing Endurance Exercise 刘 艳 ，黄元汛 ，陈筱春 ，文质君 LIU Yan ，HUANG Yuan—xun ，CHEN Xiao—chun ，WEN Zhi—j un 摘 要：目的：研究白藜芦醇对耐力训练小鼠形态、抗氧化和免疫功能的影响。方法：选用昆 明小鼠，根据体重将72只小鼠随机分为3组：A组为安静对照组；B组为耐力训练组；C组 为耐力训练+白藜芦醇组。c组小鼠每日给予白藜芦醇10rag／ 灌胃，与B组共同进行6 周递增负荷游泳训练，再进行力竭游泳实验。力竭游泳后24 h处死小鼠取材，扫描电镜下 观察各组小鼠外周血红细胞形态，测定红细胞参数，红细胞SOD活性和MDA含量，全血 GSH—Px活性，RBC— bRR和RBC—ICR。结果发现，6周耐力训练后，白藜芦醇组小鼠红细胞 畸形率显著低于耐力训练组(P0．05)；Hb、RBC和 Hct都显著高于耐力训练组(P 0．05)；红细胞MDA含量显著低于耐力训练组(PO．05)，但红细胞S()D和全血GSH—Px活 性与耐力训练组相比均无显著影响(P0．05)，RBC— bRR和RBC—ICR显著高于耐力训练 组(PO．05)。以上结果提示，白藜芦醇在一定程度上能提高耐力训练小鼠红细胞的抗氧 化能力和免疫功能，具有保护耐力训练小鼠红细胞的形态和功能的作用。 关键词：白藜芦醇；红细胞形态；抗氧化；红细胞免疫黏附功能；耐力训练；鼠；动物实验 Abstract：The purpose of this study is to investigate effects of resveratrol on morphology，anti— oxidation and immunity capacity in mice performing endurance exercise．7 2 Kunming mice were randomly divided into three groups according tO weight：group A (Sedentary Contro1)，group B(Endurance exercise)and group C (Resveratrol+ Endurance exercise)．Administrated of resveratrol with 10 mg／kg／d，the mice of group C were taken incremental load protocol of swimming for six weeks，then had an exhaustive swimming as did the mice of group B．All mice were sacrificed and sampled 24 h later．The following parameters i