羧甲基壳聚糖对大鼠胃溃疡抗氧化作用研究.pdfVIP

2007年第2l卷第6期Med J of Communications,2007,Vo1．21．No．6 · 629· [文章编号]1006—2440(2007)06-0629-03 羧甲基壳聚糖对大鼠胃溃疡抗氧化作用研究 赵健亚 吴金彪 徐广飞 (南通大学公共卫生学院营养与食品卫生教研室，江苏226001； 宁波市检验检疫局) 摘要 目的：探讨羧甲基壳聚糖对大鼠乙酸型胃溃疡的保护作用及可能机制。方法：将40只SD大鼠随机分 为正常组、模型组、硫糖铝组和羧甲基壳聚糖组。用20％Z~酸0．05ml浆膜下注射法建立大鼠慢性胃溃疡模型，手 术后第3天开始灌胃给药，连续 14天后计算各组溃疡面积，同时检测胃粘膜组织中超氧化物歧化酶(SOD)活性 和丙二醛(MDA)含量。结果：羧甲基壳聚糖、硫糖铝治疗后，乙酸诱导的溃疡面积缩小；羧甲基壳聚糖能提高大鼠 胃粘膜SOD活性，抑制MDA的升高，其作用与硫糖铝相当。结论：羧甲基壳聚糖对大鼠乙酸型胃溃疡有一定保护 作用．可能与它能提高胃粘膜抗氧化能力有关。 关键词 胃溃疡模型；羧甲基壳聚糖；超氧化物歧化酶；丙二醛 中图分类号 R151．2 Study on the antioxidant activity of Carboxymethyl-chitosan in gastric ulcer rats ZHAO Jianya，WU Jinbiao，XU Guangfei(Department of Nutrition and Food Hygiene，School of Public Health，Nantong University， Jiangsu 226001) Abstract Objec five：To investigate the protective role and its influential factors of Carboxymethyl-chitosan (CM-Chitosan) against acetic acid-induced chronic gastric ulcers in rats．Methods：40 Sprague—Dawley rats were randomly divided into normal group， model group，sucralfate group and CM-chitosan group．The rat models of chronic gastric ulcers were established by injecting 20％ acetic acid 0．05ml into gastric mucosa．14 days after the administration，rats were killed．The ulcer area was measured，and the contents of superoxide dismutase(SOD)and malondialdehyde(MDA)in gastric mucosa were detected．Results：CM-chitosan reduced the ulcer area of ulcer rats induced by acetic acid．CM-chitosan up—regulated the level of SOD in gastric mucosa．Th e level of MDA in gastric mucosa was decreased in CM-chitosan group．Conclusion：Our results suggest that CM—chitosan has protective effects on rats with acetic acid—induced gastric ulcers．It might reduce gastric ulcer by increasing antioxidant effects on gastric mucosa． Key words gastric ulcer models；Carboxymethyl-chitosan；SOD；