胎次对成年小鼠海马齿状回神经发生及其学习记忆的影响.DOC

周传农修改： 英文摘要 — 已修改 正文中的英文书写 — 已顺 图与表的英文 — 已顺 文献的英文书写 — 已顺 研究报告 胎次、性别对成年小鼠海马齿状回神经发生及学习记忆的影响 丁艳平1*，董辉2，刘知东 2，赵倩楠1 （1西北师范大学生命科学学院 2兰州大学 生命科学学院，兰州 730070） 【摘要】目的 探讨胎次、性别是否对成年小鼠海马齿状回神经发生及学习记忆产生影响。 方法 运用Morris水迷宫系统检测第1至3胎成年小鼠的学习记忆能力，腹腔注射BrdU，标记神经干细胞，检测不同胎次、性别小鼠海马齿状回中的神经发生的差异。结果 （1）在同性别、不同胎次成年小鼠间，第2胎的学习记忆能力（LMA）均显著地高于第1、3胎的，其影响规律为LMA2 LMA1 LMA3，且P 0.05；在同胎次、不同性别成年小鼠间，雌性小鼠的LMA均高于雄性小鼠的，但其差异均不显著（P 0.05）。（2）在同性别、不同胎次成年小鼠间，第2胎海马DG新生神经细胞的数量（N）均高于第1、3胎的，其影响规律分别为NF2 NF3 NF1和NM2 NM1 NM3，但其差异均不显著（P 0.05）；在同胎次、不同性别成年小鼠间，雌性小鼠的N均高于雄性小鼠的，但其差异也均不显著（P 0.05）。结论 胎次、性别对实验动物神经发生及学习记忆等方面产生的影响是肯定的。因此，在使用实验动物时，应予以充分考虑，尽量使用胎次、性别相同的。 【关键词】胎次；性别；神经再生；学习记忆；BrdU 【中图分类号】Q95-3； 文献标识码：A Effects of parity and gender on the neurogenesis of the hippocampal dentate gyrus and learning and memory ability in adult mice DING Yan-ping1*，，，Abstract] Objective To evaluate the effects of parity and gender on the neurogenesis of hippocampal dentate gyrus and the ability of learning and memory of adult mice. Methods Using the Morris water maze was used to assess the differences of learning and memory ability between the adult mice born in the first to third parities. The differences of neurogenesis were examined with BrdU-labeled hippocampual dentate gyrus of the adult mice. Results（1）The learning and memory ability (LMA) of mice born in the 2nd parity was significantly higher than that of the 1st and 3rd parity（P0.05） in the same sex, that was LMA2 LMA1 LMA3 . Besides, female mice had better learning and memory ability than the male mice, (P0.05). (2) The number of BrdU-positive cells of the mice born in the 2nd parity was higher than that of the 1st and 3rd parity in the same sex, but the difference was non-significant (P 0.05), and BrdU-positive cells of the female mice was higher than that in the male mice, but also with a　non-significant　difference. Conclusions There are essential difference in the neurogenesis of hippocampal dentate gyrus and the learning and memory ability in adult mi