两种不同基因型的白叶枯病菌对菜豆叶片叶绿素荧光参数的影响.doc

油菜黄单胞杆菌AhpC突变对被侵染菜豆叶片光化学反应特性的影响* 贾凌云1，焦青松1 ，李欣2 ，孙坤1*，冯汉青1 （1.西北师范大学生命科学学院，兰州 730070；2.河南科技大学食品与生物工程学院，洛阳，471000） 摘要：本文研究了两种不同基因型的黄单胞杆菌[野生型(Xcp-W)和烷基过氧化物还原酶亚基C（AhpC）突变体(Xcp-ahpC)]侵染菜豆叶片(Phaseolus vulgaris. L)后，叶片侵染位点以及同一叶片上距侵染位点不同距离处叶绿素荧光参数的变化。结果显示，对照相比，两种病原菌侵染后叶片被侵染位点以及同叶距侵染位点一定距离处潜在最大光化学效率(Fv/Fm) 的变化不显著，而光系统Ⅱ(PSⅡ)实际光化学效率(Y())、电子传递速率(ETR)以及光化学猝灭系数(qP和qL)均显著降低。与野生型病原菌Xcp-W的侵染相比，Xcp-ahpC突变型菌株侵染后叶片的以上参数的降低更为明显。野生型病菌Xcp-W侵染对非调节性能量耗散产量(Y(NO))和调节性能量耗散产量(Y(NPQ))无显著影响；但Xcp-ahpC型病原菌侵染则导致了 (Y(NO))和(Y(NPQ))的显著上升。综上可知，白叶枯病菌侵染使得叶片光系统Ⅱ的光能使用效率受到了系统性的抑制，而病原菌烷基过氧化物还原酶亚基C对叶片光系统Ⅱ的光能使用效率的抑制作用更为强烈，并影响了植物的光能耗散机制。 ＡThe effects of the infection with Xanthomonas campestris pv. Phaseoli with the mutation in ahpC on chlorophyll fluorescence characteristics of bean leaves JIA Ling-Yun1 ,JIAO Qing-Song1 , LI Xin2 , SUN Kun*1 , FENG Han-Qing1 (1.College of Life Sciences, Northwest Normal University, Lanzhou 730070, China; 2.Food and Bioengineering College, Henan University of Science and Technology, Luoyang 471000, China) Abstract：After the bean (Phaseolus vulgaris L.) leaves were infected with two different genotypes of Xanthomonas campestris pv. Phaseoli, either wild-type (Xcp-W) or the mutant lacking Alkyl hydroperoxide reductase subunit C (Xcp-ahpC), the changes of the chlorophyll fluorescence characteristics in both the infected site and the locations distant from the infected sites were studied. The results showed that in the infected site and the locations distant from the infected sites, the potential maximal photochemical efficiency (Fv/Fm) did not significantly change, while photosynthesis system Ⅱ (PSⅡ), actual photochemical efficiency (Y(Ⅱ)), electronic transmission rate (ETR), and the coefficient of photochemical quenching (qP) were significantly decreased. The decreases of PSⅡ, Y(Ⅱ), ETR, and qP in the leaves infected with Xcp-ahpC were more drastic than those in the leaves infected with Xcp-W. The quantum yield of non-regulated energy dissipation (Y(NO)) and quantum yield of reg