氮水添加对半干旱地区草原土壤微生物群落生理功能的交互影响.doc

DOI: 10.3724/SP.J.1011.2012.00000 增雨和施氮对半干旱地区草原土壤微生物群落碳源利用潜力的影响 毕 捷1 张乃莉2 梁 宇2 蔡 禄1 马克平2 (1. 内蒙古科技大学 包头 014010; 2. 中国科学院植物研究所 北京 100093) 已有许多研究证明, 中国北方草地生态系统的植物群落结构和组成对气候变化和氮沉降较为敏感, 但是关于草原土壤微生物群落方面的研究较薄弱, 特别是有关土壤微生物群落对多重环境因子变化的响应方面的研究较少。水和氮元素是陆地生态系统生产力的两大限制性因子。本研究在内蒙古多伦半干旱草原地区进行增雨和施氮的野外控制试验, 以模拟未来该地区的降水变化和氮沉降, 使用碳源利用水平剖面分析法, 监测样地土壤理化指标和土壤微生物群落碳源利用潜力的变化。通过三年的跟踪监测, 试验结果显示: 增雨显著提高了土壤含水量和有机质含量, 增雨同时施氮则显著提高了土壤可溶性氮含量, 施氮降低了土壤pH值。试验表明: 在半干旱草原地区, 单独施氮和单独增雨都没有引起土壤微生物群落碳源利用潜力的显著变化, 而在同时增雨和施氮条件下, 微生物群落对6大类碳源利用潜力的响应呈现双向正效应, 说明在水和氮都充足的条件下, 土壤微生物碳源利用潜力才会显著提高。这预示着在未来降雨增加和氮沉降的全球变化背景下, 中国北方半干旱草地生态系统的碳循环速率可能会提高。 关键词　土壤微生物 碳源利用潜力 施氮 增雨 半干旱草原中图分类号: Q938.1+3 文献标识码: A 文章编号: 1671-3990(2012)1-0000-08 Impacts of N addition and increased precipitation on microbial C utilization potentials in a semiarid steppe BI Jie1, ZHANG Nai-Li2, LIANG Yu2, CAI Lu1, MA Ke-Ping2 (1. Inner Mongolia University of Science Technology, Baotou 014010, China; 2. Institute of Botany, the Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China) Abstract Plant community structure and composition were quite sensitive to climate change and N deposition in semiarid grassland of northern China, yet there were few research on the responses of microbial community. The aim of the study is to assess variations in soil microbial communities under future scenarios of changing precipitation and N deposition in a semiarid grassland of northern China. Soil microbial community microbial C utilization potential were determined by community-level physiological profiles in a 4-year field experiment with water and N additon. Either increased precipitation or N addition had changed soil environment, which affected microbial C utilizations. There were bilateral positive interactions between water and nitrogen in affecting microbial C utilization potentials, which implies that microbial C utilization potentials tend to be inactive until both N and water availability are sufficient. The microbia