细胞生物学的研究思路.pptVIP

细胞生物学研究思路的探讨;研究背景;神经嵴发育不全：由于神经嵴细胞（neural crest cells，NCC）发育缺陷或障碍而导致神经嵴出现异常的增殖、生存、迁徙和分化。由于NCC发育不良导致的这些组织和细胞的病变统称为神经嵴病。 ;研究背景;;研究背景;基因组水平：WS致病基因突变检测;研究内容; 课程提纲 ;蛋白质加上标签 (Tag); 课程提纲 ;定点突变 site-directed mutagenesis;突变引物设计;; 课程提纲 ;转染-transfection;Transfection methods;DEAE-dextran Diethylaminoethyl (DEAE)-dextran is one of the oldest methods for introducing nucleic acids into cultured mammalian cells. The positively charged DEAE-dextran molecule interacts with the negatively charged phosphate backbone of the nucleic acid. The DNA–DEAE dextran complexes appear to adsorb onto the cell surface and be