星点设计-效应面法优化壳聚糖温敏凝胶的制备及缓释性考察.docVIP

星点设计-效应面法优化壳聚糖温敏凝胶的制备及缓释性李晓娟郭亚可，，陈文( (新疆石河子大学药学院，新疆 石河子 832000) 摘要目的壳聚糖Chitosan/β-Glycerophosphate, CS/β-GP）温敏凝胶，为缓释提供基础。方法（degree of deacetylationDD）、质量、pH，选取pH为星点设计考察因素，胶凝时间为考察指标，对结果进行多元线性和多项式拟合，效应面法优化工温敏凝胶结果因素与指标之间的最佳三次多项式最 mg·mL-1，pH为7.08，胶凝时间为6.23 min。且释药行为接近Weibull结论星点设计效应面温敏凝胶关键词星点设计；效应面法；温敏凝胶；神经生长因子 Study on preparation and sustained-release of chitosan thermosensitive hydrogel by central composite design and response surface methodology LI Xiao-juan , GUO Ya-ke, HAN Bo, CHEN Wen (School of Pharmacy, Shihezi University, Shihezi, Xinjiang, 832000, China) [Abstract] Objective: The purpose was to optimize the preparation process of chitosan/β-glycerophosphate (CS/β-GP) thermosensitive hydrogel and to lay a foundation for further research of sustained-release delivery system. Methods: By examining the degree of deacetylation (DD) of chitosan, the quality of chitosan, β-GP concentration and pH, the study select the β-GP concentration and pH as investigation factors, gelling time as index, The results were to fit multiple linear or polynomial and to optimize the process by response surface methodology. The study on Sustained-release of CS/β-GP temperature-sensitive gel as a model drug of nerve growth factor (β-NGF), and explore its release model. Results: The results of the multiple correlation coefficient shows that the best-fit equation between the factors and indicators for the cubic polynomial, the optimum: the concentration of β-GP was 511.51 mg·mL-1, pH was 7.08, gel time was 6.23 min, and the release behavior was close to the Weibull model. Conclusion: The optimization of CS/β-GP temperature sensitive gel by central composite design-response surface has the advantages of short gel time and sustained-release to meet the requirements of implantable drug delivery. [Key Words] central composite design; response surface methodology; thermosensitive hydrogel; nerve growth factor; sustained-release 神经生长因子是1952年由意大利科学家丽塔?L?蒙塔尔奇尼（Rita L Montalcin）发现的第一个神经营养因子，其