电聚合修饰碳纤维电极及乳酸脱氢酶活性的测定 - 分析化学.pdfVIP

29 (FENXI HUAXUE) 12 2001 12 Chinese Journal of Analytical Chemistry 1457~ 1460 习玲玲* 施清照 (, 310031) ( , 310028) (MG) ( 7 m) , + - 4 - 3 ( LDH) (NAD ) 6.0 10 molL, 5.0 10 mol/ L pH 7.0 NaOH-KH O , + 0. 10 V , , 15~ 240 2 4 U/ mL, 10 U/ mL, 15 s , , , 1 ( LDH) , , LDH , , LDH , LDH , , LDH , 1,2 3 , , ; , 4 5 ; , , + 0. 10V 2 21 JF-01 ( ) , 1M20A- 100 ( ) , , LDH( 15 Umg, ) , MG( ) , L- + NAD ( ) , L-L- ( ) , , 22 HNO3 , , - 5 1.010 mol/ L MG, 1.0 mol/ L KNO3 , pH 7.0 NaOH-KH2 O4 , + 2. 5V 30 s, - 0. 50~ + 0. 50V 30 min, , 3 31 : 2001-01-14 ; 2001-07-12 1458 29 LDH + + L-Lactate+ NDA yruvate+ NADH+ H + MG( ox) + NADH MG( red) + NAD MG( red) MG( ox) , L-LDH , (NADH) MG , MG 32 , 1, , , ; 2 1020406080 mVs 12 , ip v 33 , (22 ) , - 3 - 6 ( MG) 10 10 ~ 1.0 10 molL , 0~ 45 min , :MG 10 10- 5 molL 30 min 34 1 MG 341 , Fig. 1 Multicyclic voltammogram of methylene green (MG) at carbon fibre electrode 3 - 0. 50~ + 0. 30 MG: 1.