荧光标记鹿茸蛋白提取物胃肠道吸收离体实验研究in vitro - 药学学报.pdfVIP

1526 药学学报 Acta Pharmaceutica Sinica 20 11, 46 ( 12) : 1526 1529 ·研究简报· 荧光标记鹿茸蛋白提取物胃肠道吸收离体实验研究 1, 2 1 2 2 1* 倩 , 胡剑江 , 周秋丽 , 王新月 , 王 毅 ( 1. 中国中医科学院医学实验中心, 北京 100700; 2. 吉林大学药学院, 吉林 长春 130021) 关键词: 鹿茸蛋白提取物; 荧光标记; 人工胃液; 人工肠液; 肠囊外翻实验 中图分类号: R965 文献标识码:A 文章编号: 05 13-4870 (20 11) 12-1526-04 In vitro study on gastrointestinal absorption of FITC labeled Pilose Antler protein extraction 1, 2 1 2 2 1* ZHANG Qian , HU Jian-j iang , ZHOU Qiu-li , WANG Xin-yue , WANG Yi (1. Exp erimental Research Center, China Academy of Chinese M edical Sciences, Beij ing 100700, China; 2. School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China) Abstract: An in vitro detection method of the gastrointestinal absorption of Pilose Antler protein was established for mixed protein activity. Five bands of protein with molecular weight of 17.8 160 kD derived from the Pilose Antler were extracted and sufficiently labeled with FITC (FITC-PE). The stability and variation of FITC-PE in gastrointestinal circumstances were detected by native polyacrylamide gel electrophoresis and confocal laser scanning microscope. Results showed that the main component of FITC-PE kept invariant after being reacted with artificial gastric fluid and artificial intestinal fluid. The fluorescence signal was detected 20 min after administration in the valgus intestinal purse experiment, and three kinds of protein, with molecular weight of 45, 25, and 17.8