KiSS-1 mRNA和MMP-9 mRNA在肺癌中的表达及意义.pdfVIP

中国医师进修杂志2008年1月5 13第3l卷第1期内科版 Chin JPostgradMed，January5th2008，Vo1．31，No．1A · 29· 临床论 著 · KiSS一1 mRNA和MMP一9 mRNA在肺癌 中的表达及意义 葛云洁 王野 姜长青 赵伟叶 黄梅青 纪霞 【摘要】目的 探讨 ss—l mRNA和MMP一9 mRNA在肺癌组织中的表达，及其在肺癌侵袭及 转移中的作用和临床意义。方法 采用逆转录聚合酶链反应(RT—PCR)技术检测56例肺癌组织及 10例正常肺组织KiSS一1 mRNA和MMP-9 mRNA表达，分析其与各种临床参数的关系。结果 肺 癌组织KiSS一1 mRNA和MMP一9 mRNA阳性率分别为30．4％和62．5％，正常肺组织分别为100．0％ 和10．0％(P0．01)。肺癌组织中KiSS一1 mRNA表达与临床分期、淋巴结转移、远处转移有关， MMP一9 mRNA表达也与肺癌的淋巴结转移和远处转移有关。而KiSS一1 mRNA阳性的肺癌组织 MMP一9 mRNA阳性率为35．3％，而KiSS一1 mRNA阴性肺癌组织中MMP一9 mRNA的阳性率为 74．4％，KiSS一1 mRNA和MMP一9 mRNA的表达呈负相关 r=一0．371，P0．01 o结论 KiSS一1失表 达与肺癌的侵袭和转移密切相关，KiSS一1可能通过抑制MMP一9合成而抑制肺癌的侵袭转移。 【关键词】肺肿瘤；KiSS一1；MMP-9；肿瘤转移 Expression and significance of KISS-1 mRNA and MMP-9 mRNA in lung cancer GE Yah- ， WANG Ye，JIANG Chang-qing，ZHA0 Wei--ye，HUANG Mei—qing，JI Xia．Department ofRespira— tion，QingdaoMunicipalHospital，Qingdao 266011，China 【Abstract】Objective To investigate the expression and clinical significance of KiSS一1 mRNA and MMP-9 mRNA in the invasion and metastasis oflung cancer．Me~ods The expressions of KISS-1 mRNA and MMP-9 mRNA in 56 patients with lung cancer and 10 patients with normal lung tissues were detected by reverse transcriptase—polymerase chain reaction (RT—PCR)，and its relationships with clinical parameters were analyzed．Results The positive rates of KISS-1 mRNA in lung cancer and normal lung tissues were 30．4％and 100．0％respectively (P0．01)．The expression of KISS-1 mRNA in patients with lung cancer Was correlated with its clinical stage，lymph node metastasis and distant metastasis．The positive rates of MMP-9mRNAinlungcancerand normallungtissueswere 62．5％and 10．0％respectively(P0．01)．The expression of MMP-9 mRNA in patients with lufig cancer was correlated with its lymph node metastasis and distantmetastasis．Inlungcancer，thepositive rate ofMMP-9mRNAwiththe expression ofKISS-1 mRNAin lung