α-SMA在口腔粘膜下纤维性变成纤维细胞中的表达.pdfVIP

李 霞等 一SMA在口腔粘膜下纤维性变成纤维细胞中的表达 第9期 ~-SMA在口腔粘膜下纤维性变成纤维细胞中的表达 李 霞① 凌天牖 高义军 (湖南长沙中南大学湘雅二医院口腔科，长沙41001 1) 中国图书分类号 R781．5 文献标识码 A 文章编号 1000-484X(2007)09-0851-06 [摘 要] 目的：探讨肌成纤维细胞在口腔粘膜下纤维性变(OSF)发病机制中的作用。方法：免疫组化法检测组织中和 体外培养成纤维细胞中平滑肌肌动蛋白 ( —SMA)的表达；免疫组化法和RT—PCR法检测槟榔碱对OSF及正常成纤维细胞 产生 —SMA和其mRNA表达情况。结果：正常口腔粘膜组织中除血管壁外无阳性染色，而OSF组织中在粘膜下层有大量梭 形细胞胞浆被染成棕黄色；体外培养的正常组织来源成纤维细胞中有很少量的 —SMA蛋白表达，OSF来源的成纤维细胞中早 期有大多数细胞(85．80％±3．56％)表达 —SMA；槟榔碱干预成纤维细胞后不能增加平滑肌肌动蛋白 蛋白和mRNA的表 达。结论：肌成纤维细胞可能在OSF的发病机制中起重要作用，OSF组织中的肌成纤维细胞可能不是槟榔成分直接作用于成 纤维细胞转化而来的。 [关键词] 口腔粘膜下纤维性变；肌成纤维细胞；平滑肌肌动蛋白 ；槟榔碱 Expression of a-SMA in fibroblasts of oral submucous fibrosis L／Xia，LING Tian—You，GAO Yi—Jun．Department of Stomatology，Second Xiangya Hospital，Central South University， Changsha 41001 1，China [Abstract] Objective：To explore the effects of myofibroblast in the pathogenesis of oral submueous fibrosis(OSF)．Methods： The expressions of —SMA in the tissues of OSF and normal control were detected by immunohistochemistry．Fibroblasts were isolated from oral mucosa of OSF patients and healthy controls and were cultured under stimulation by arecoline．The expressions of —SMA in OSF fibroblasts and normal fibroblast were detected by immunocellchemistry and RT—PCR．ResllIts：(1)No positive expression was found in normal tissues except in the blood vessel，but in OSF tissue， —SMA positive expression was found in many spindle ceils in the oral submucousal layer．(2)Few fibroblasts in vitro from normal controls were Ot—SMA positive．(3)Most of the second passage of OSF fibroblasts were Ot—SMA positive(85．80％±3．56％)．(4)The expression of —SMA in fibmblasts could not be increased by the stimu— lation of arecoline．Conclusion：Myofibroblasts may play an important role in the pathogenesis of OSF，and the appearrence of myofibro— blasts in OSF may not be directly induced by the stimulation of arecoline． [Key words] Oral submucous fibrosis；Myofibroblast；Ot—SMA；Arecoline 口腔粘膜下纤维性变(Oral s