碳源和氮源对绿粘帚霉合成几丁质酶的影响-四川农业大学学报.pdf

DOI :10.16036/j.issn.1000 -2650.2006.01.006 24 　 1 Vol.24 o.1 2006 3 　　　　　　　　 　Journal of Sichuan Agricultural University 　　　　　　　　 　 Mar.2006 Effect of Carbon Sources and Nitrogen Sources on Chitinase Production by G .virens 1 1 1 1 2 QIA O T ian-min , Z HU T ian-hui , L I F ang-lian , Chen Y ao , DE NG Y ue-ping (1.College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China; Forestry Bureau of anchong City, anchong 637000, Sichuan, China) Abstract:The chitin is the reborn and second natural resource in the nature and just next to the cellu- lose.The chitinase has very important function in degrading chitin and preventing plant disease.Glio- cladium virens is a very important antagonistic fungus in biological control.This paper used different carbon sources such as colloid chitin, chitin-powder, glucose, sucrose and different nitrogen sources such as K O , (H )SO , peptone, yeast-decoction to study the chitinase production by G.virens 3 4 2 4 F051.The favorable carbon source and nitrogen source of producing high chitinase were chitin-powder and yeast-decoction. Key words:chitinase;Gliocladium virens;carbon source;nitrogen source 1 1 1 1 2 谯天敏, 朱天辉 , 李芳莲 , 陈　瑶, 邓月平 (1.四川农业大学 林学园艺学院, 四川雅安　625014;　2.四川省南充市林业局, 四川南充　637000) :几 丁质是自然界中仅次于纤维素 第二大可再生自然资源, 几 丁质酶在降解几 丁质方面有重要功能, 而且在 植病防治方面有重要作用。 绿粘帚霉(G.virens)是生物防治中具有前途 拮抗菌。 本文以胶体几 丁质、细粉几 丁 质、葡萄糖、蔗糖为不同碳源, 以K O 、(H )SO 、蛋白胨、酵母浸粉为不同氮源对绿粘帚霉F051 合成几 丁质酶进 3 4 2 4 行初步研究, 发现绿粘帚霉F051合成几 丁质酶 最佳碳源