02-6植物病毒探究.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * A close look at the structure of Tobacco Mosaic Virus.TMV is made up of a spiral or helix of? 2,130 protein (CP) subunits. The TMV RNA, indicated in red, is about 6400 nucleotides in length. During assembly of the TMV particle, the interaction between the CP subunits and the RNA results in a rigid helical rod of ca. 300 nm x 18 nm. (A) Ten turns are illustrated in a side view of a small portion of a TMV particle (B). The maximum diameter of the subunit particle is 9 nm, and the mean diameter is 7.5 nm. The hollow core down the center is 4 nm in size. [Note: 10A = 1 nm] By contrast, sixty protein subunits are packed to form the protein shell of an icosahedral virus particle such as Tomato bushy stunt virus (C). The rotation symmetry is the same as the icosahedron illustrated (D).? The RNA arrangement in the center of these T=3 icosahedral viruses is unknown. * * * * * * Figure 8. Tobacco mosaic virus (TMV) genomic and subgenomic RNAs. (A) The 6400 nucleotide TMV genomic RNA acts as a messenger RNA for the expression of the 126 kDa and 183 kDa replicase-associated proteins. (B) The other genes on TMV RNA are expressed from subgenomic RNAs (sgRNAs) during the replication cycle (see Figure 9 for details). The movement protein (MP), the coat protein (CP), and replicase proteins (RP) have been detected in plants infected with TMV. The 54 kDa-sgRNA accumulates in TMV-infected plants, but thus far the predicted 54 kDa protein has not been detected. The rod-shaped TMV virions are composed of the CP and the genomic RNA (see Figures 6 and 7). (Courtesy K-B.G. Scholthof) * * * * * * * * * * * * * * * 3 植物病毒的分类及主要属 3.1 植物病毒的分类 权威机构为国际病毒分类委员会 (International Committee on Taxonomy of Viruses, ICTV) 植物病毒分会 近代病毒分类体系趋于将病毒单列为“病毒界” 根据核酸类型可将植物病毒分为五大类： +ssRNA、-ssRNA、dsRNA、ssDNA、dsDNA。 ICTV 第六次报告 (1995) ： 729种植物病毒，分为9个科，47个属 基本明确科、属、种的关系 3.2 植物病毒的命名 不采用拉丁文双名法进行命名 种名以寄主名称加上症状来命名 Tobacco mosaic virus (