生物物理2概要1.pptVIP

五、蛋白质折叠/去折叠的实验研究方法 Fast/Ultra fast mixingStopped-flow, quench flow, continuous flow,.. T-jumpelectrical-discharge-induced T-jumpLaser-induced T-jump OthersLaser flash photolysisMechanic unfolding…… Stopped-flow 典型停流实验结果 Continuous-Flow 时间分辨CD Quench-flow H/D exchange T-jump Mixing Electrical-discharge-induced T-jump Laser-induced T-jump Rise 1-20?C in 500 ns-10 ?s Laser-induced T-jump Initiation of protein folding may be extended down to about 20 ps for a 10 kDa protein Laser flash photolysis Photodissociation takes less than 100 femtoseconds! Mechanic unfolding with AFM * Anfinsen实