二氧化碳气腹对人卵巢癌细胞系SKOV3裸鼠腹腔转移瘤生长的影响及机制探讨.pdfVIP

重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 21108．Vo1．33 No．4) 一 441一 文章编号：0253—3626(2008)04—0441—05 二氧化碳气腹对人卵巢癌细胞系SKOV3裸鼠腹腔转移瘤生长 的影响及机制探讨 唐均英，黄 金，姚珍薇 (重庆医科大学附属第一医院妇产科，重庆 400016) 【摘 要】目的：研究二氧化碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响，并初步探讨其机制。方法：构建裸鼠腹腔转移瘤 的动物模型，模拟腹腔镜C02气腹环境，通过观察肿瘤种植生长的情况，及TUNNEL法测定肿瘤组织凋亡情况，以探讨二氧化 碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响；通过免疫组织化学法和Western—blot测定肿瘤组织中VEGF及MMP-9表 达，以探讨其可能存在的机制。结果：经CO 气腹处理后，腹腔内肿瘤质量大于对照组，肿瘤组织中VEGF和MMP-9蛋白的表 达差异无显著性，各组间肿瘤细胞的凋亡率差异无显著性。结论：c0 气腹有促进卵巢肿瘤细胞SKOV，生长的作用。 【关键词】CO2气腹；裸鼠；凋亡；VEGF；MMP一9 【中国图书分类法分类号】R737．31；R656 【文献标识码】A 【收稿日期12007—10-26 Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line(SKOV3)in nude mice and analysis of its mechanism TANG Jun-ying，et ol (Department of Gynecology and Obstetrics，the First Affiliated Hospit01，Chongqing Medical University) 【Abstract】Objective：To investigate the effects of carbon dioxide(CO2)pneumoperit0neum on the abd growth of ovarian carcinoma cell in nude mice and to primarily analyze its mechanism．Methods：The nude mice model of abdomino-metastatic tumor of ovarian carcinoma cell was established， and laparoscopic CO2 pneumoperitoneum environment was simulated．Tumor implantation and growth were observed and tumor tissue apoptosis was detected by TUNNEL method，to explore the effect of CO2 pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice． The expressions of VEGF and MMP9 in cancer tissue were determined by immunohistochemistry and Western Blot to analyze the possible mechanism．Results：After the treatment of CO2 pneumoperitoneum，the tumor quality in abdominal cavity was higher than that of the control group，the expression difference of VEGF and MMP9 in tumor tissues