abcam：Indirect flow cytometry (FACS) protocol.docxVIP

Indirect flow cytometry (FACS) protocolGeneral procedure for flow cytometry using a primary antibody and conjugated secondary antibodyIndirect labelling requires two incubation steps, firstly with a primary antibody then with a compatible secondary antibody. The secondary (and not the primary) antibody has the fluorescent dye (FITC, PE, Cy5, etc.) conjugated. Please note that this is a general protocol and you may need to adapt it for your applications.General Procedure:1. Harvest and wash the cells then determine the total cell number.Cells are usually stained in polystyrene round bottom 12 x