肉苁蓉抗运动性疲劳机制的实验研究.docVIP

　　肉苁蓉抗运动性疲劳机制的实验研究 【摘要】 ：目的 观察肉苁蓉对负重游泳小鼠肝脏乳酸脱氢酶同工酶、糖原及一氧化氮合酶3(NOS3)的影响,探讨其抗运动性疲劳的作用机制。方法 将昆明种小鼠随机分为正常对照组、运动对照组和肉苁蓉实验组。正常对照组和运动对照组每只每日灌胃生理盐水0.2 mL,肉苁蓉实验组每只每日灌胃肉苁蓉水煎液0.2 mL(3 g/kg),共15 d；末次给药1 h后,运动对照组和肉苁蓉实验组小鼠尾部负重5%游泳90 min,10 h后连同正常对照组小鼠处死取肝脏,一部分用中性甲醛固定制备石蜡切片,一部分制成肝细胞匀浆检测乳酸脱氢酶活性；常规HE染色观察肝脏组织结构,糖原染色法显示肝脏糖原,S-P免疫组织化学染色法检测小鼠肝脏NOS3的表达。结果 运动对照组与正常对照组比较,乳酸脱氢酶活性高(Plt;0.05),肝脏结构损伤严重,糖原含量减少,NOS3表达下降(Plt;0.05)；肉苁蓉实验组与运动对照组比较,乳酸脱氢酶活性降低,主要是乳酸脱氢酶5(LDH5)下降,肝小叶结构较清晰,糖原丰富,NOS3表达上调(Plt;0.05)。结论 肉苁蓉可降低LDH5活性,上调NOS3的表达,促进肝糖原合成,发挥肝脏保护和促进体能恢复作用。 【关键词】 肉苁蓉　肝糖元　一氧化氮合酶3　小鼠 　 Abstract：Objective To study the effect of cistanche deserticola on lactate dehydrogenase (LDH) isoenzyme, glycogen and nitric oxide synthase 3 (NOS3) of liver in the mice burden sming and explore relevant molecular mechanisms of anti-sports fatigue. Methods The mice al control group, the sport control group and the cistanche deserticola experimental group. Each mice of the normal control group and the sport control group L per day. Each mice of the cistanche deserticola experimental group L (3 g/kg) per day. The administrations ming for 90 minutes ice of the sport control group and the cistanche deserticola experimental group at 1 hour after the last administration. Livers of the mice oved after 10 hours of sming. One part of liver alin liquid to prepare the paraffin sections and the others easurement of LDH activity. The structure of the liver easured by staining of glycogen. NOS3 ined by S-P immunohistochemical method. Results The liver structure of the sport control group e of LDH4 and LDH5 al control group (Plt;0.05). The liver structure of the cistanche deserticola experimental group e ice burden sming and accelerate glycogen accumulation by increasing expression of NOS3 to protect liver and improving physical capacity recovery. 　　Key ice 　肉苁蓉,又名大芸,为列当科二年寄生草本植物,干燥带鳞叶的肉质茎,主产于内蒙古、甘肃、新疆、青海等地的沙质土壤和半沙质的草原地带,在众产地中以内蒙肉苁蓉质量最佳。肉苁蓉性味甘、咸、温,入肾、大肠经,主要功用是补肾壮阳、益精血、润肠通便,常用于治疗阳痿、不孕、腰膝酸软、筋骨无力、肠燥便秘等病症。本研究旨在通过小鼠负重游泳试验,观察肉苁蓉对负重游泳小鼠肝