运动对慢性疲劳综合症小鼠NK细胞活性和IL-2水平的影响.pdfVIP

第36卷第1期 南华大学学报·医学版 2008年 1月 Vo1．36 No．1 Journal of Nanhua University(Medical Edition) Jan．2008 运动对慢性疲劳综合症小鼠NK细胞 活性和IL一2水平的影响 唐双阳 ，李 乐 ，陈 熙 ，胡四海 ，余敏君 ，黄祁平 ，万艳平 (1．南华大学病原生物学研究所，湖南衡阳421001；2．南华大学公共卫生学院) 摘 要：目的 建立慢性疲劳综合症(CFS)小鼠模型，研究运动对CFs小鼠NK细胞活性和IL一2 水平的影响。方法 选取40只小鼠随机分为4组，每组l0只。I组为正常对照组，Ⅱ组为CFS对照 组，Ⅲ组为CFS运动1组，Ⅳ组为CFS运动2组。用力竭性游泳试验建立CFS小鼠模型。不同强度运动 后，计算脾脏指数，M1Tr法和EL1SA分别检测小鼠脾脏中NK细胞活性与lIJ一2含量。结果 脾脏指数 试验组明显低于CFs对照组(P0．05)；每天运动两次可明显升高CFS小鼠NK细胞活性和增加脾脏 一 2诱生量，与c 对照组比较，差异有显著性(P0．05)，且随运动量增加可增强NK细胞活性、提高 IL一2水平。结论 适当运动能提高CFS小鼠的NK细胞活性和IL一2含量。 关键词：运动； 慢性疲劳综合症(ces)； NK细胞； IL一2 中图分类号：R37 文献标识码：A 文章编号：1672—7444(2008)01—0012—03 Effects of Exercises on IL一2 Contents and NK Cell Activities of Murine Models With Chronic Fatigue Syndrome TANG Shuang—yang，LI le，CHEN Xi，et al (Institute ofPathogenic Biology，Nanhua University，Hengyang Hunan 421001，China) Abstract：Objective To build Murine models with chronic fatigue syndrome(CFS)and investigate the effects of exercises on IL一2 and NK Cell activities of Murine models with CFS． Methods Fon)r mice were randomly divided into four groups aS Group I(comparative normal group，10 mice)，Group II(comparative CFS group，10 mice)，Groupm(exercise 1 group，10 mice)，Gmup]~(exercise 2 group，10 mice)．The Murine models with CFS were built by exhaustive swimming．After murine models were exercised by two kinds of intensity，their spleen in— dexes were calculated．The NK Cell activities were measured by Mr丌 assay and the contents of splenic IL一2 were determined by ELISA，respectively． Results The spleen index of Exercise Groups were significantly low，and diem was obvious difference compared with GI。oup 1I(P0．05)．The contents of splenic IL一2 and the NK(；ell Acti~ ities i