微生物菌群之间配比协调关系的研究.pdf

DOI牶牨牥牣牨牭牴牫牫牤j牣cnki牣牨牥牥牬牠牫牪牰牳牣牪牥牥牱牣牥牴牣牥牫牫 2007 年第9 期 1 2 2 3 4 2 * 郭金玲, 李　炜 ,郑秋红 ,刘海奎 , 王国强 ,尹清强 (1. , 450011;2. , 450002; 3. , 475003;4. , 473000) :对乳酸杆菌、乳酸球菌、枯草芽胞杆菌、酵母菌、大肠杆菌等菌种进行单独培养和不同比例的 混合培养, 测 了发酵过程中pH 值、活菌数量及光密度的变化情况。结果表明,与含有25 %混合 菌液的发酵麸皮相比,含50 %混合菌液的发酵麸皮, 在发酵24 h 后的需氧或厌氧活菌总数都有升 高趋势(P0. 05), 发酵1 周后其pH 值的下降趋势明显(P0. 05), 说明乳酸菌数越多使 pH 下降 越大。利用需氧(LB)和厌氧(M RS )培养液分别交叉培养需氧菌和厌氧菌,均能在相应的培养基上 生长。充分说明厌氧菌和需氧菌混合培养在理论上是可行的。但是, 如果用滤菌膜过滤后的培养 过乳酸菌的M RS 培养基来培养需氧菌时,这些菌种均不能在滤液中生长。 :微生物;混合发酵;pH 值;光密度 + :Q93 . 1 5　　:A 　　:1004-326 (2007)09-0106-04 Study on the Proportion and Coordination of Microorganisms 1 2 2 GUO Jin-ling , LI Wei , ZHENG Qiu-hong , 3 4 2 * LIU Ha-i kui , WANG Guo-qiang , YIN Qing-qiang (1. Department of Animal Husbandry, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China;2. College of Animal Science and Veterinary Engnigeering, Henan Agricultural University, Zhengzhou 450002, China;3. Henan Food and Oil T rade Company, Kaifen 475003, China;4. Nanyang Agricultural College, Nanyang 473000, China) Abstract:L actobacil l us,L actococcus,B aci l lus subt i lis , Yeast, E . col i w ere pure cultured and co- cultured to determine their proportion and coordination by estimating the changes of pH value, viable count and optical density. The results indicated that the aerobic or anaerobic viable colonies were increased(P0. 05)in fermented w heat bran mixed with 50%bacterium culture after 24h fermentation, compare