短刺小克银汉霉对马钱子生物碱盐转化工艺的研究 - 天津中草药杂志社.pdf

中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 23 期 2013 年 12 月 ·3309 · 短刺小克银汉霉对马钱子生物碱盐转化工艺的研究 1 2 1* 潘自皓 ，金 苗 ，潘 扬 1. 南京中医药大学药学院，江苏 南京 210046 2. 中国科学院南京地理与湖泊研究所，江苏 南京 210008 摘 要：目的 为提高短刺小克银汉霉对马钱子中马钱子碱与士的宁的转化率，构建高效的短刺小克银汉霉微生物转化体系。 方法 根据菌种的生长代谢规律，采用单因素实验法，以马钱子碱硫酸盐和士的宁硝酸盐作为底物，以对底物的转化率作为 指标，对可能的影响因素进行了考察。结果 获得了优化的转化工艺，即接种量为 4% 、发酵时间为2.5 d 、底物质量浓度为 20 mg/L 、转化时间为 3 d、培养温度为28 ℃、培养基初始pH 值为 6.5、摇床转速为 150 r/min。在此工艺条件下，2 种底物 的平均转化率分别达到了77.75%与 77.10% 。结论 短刺小克银汉霉对 2 种底物的平均转化率分别提高了约 17%与 22% ，此 微生物转化体系能够满足马钱子减毒存效研究的需要。 关键词：短刺小克银汉霉；马钱子碱；士的宁；微生物转化；减毒存效 中图分类号：R282.15 文献标志码：A 文章编号：0253 - 2670(2013)23 - 3309 - 07 DOI: 10.7501/j.issn.0253-2670.2013.23.010 Study of microbial transformation process of Strychnos nux-vomica alkaloid salts by Cunninghamella blakesleeana 1 2 1 PAN Zi-hao , JIN Miao , PAN Yang 1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China 2. Nanjing Institute of Geography Limnology, Chinese Academy of Sciences, Nanjing 210008, China Abstract: Objective To enhance the transformation rate of brucine and strychnine in Strychnos nux-vomica and establish a high efficient microbial transformation system by Cunninghamella blakesleeana. Methods Based on the growth and metabolic rule of C. blakesleeana, through single-factor experiments, by using brucine sulphate and strychnine nitrate as substrates and the transformation rate of each substrate as index, several influential factors were investigated. Results The optimal transformation condition was inoculation amount at 4%, fermentation time at 2.5 d, substrate concentration at 20 mg/L, transformation time at 3 d, culture temperature at 28 ℃ , initial pH of medium at 6.5, and shaking speed at 150 r/min. Under the above condition, the average transform