生防菌混合培养液对烟草普通花叶病毒的抑制作用 - 中国烟草科学.pdf

110 Chinese Tobacco Science 2013-06 ，34 （3 ） * 266101 Tobacco mosaic virus, TMV TMV 5 Ba CZA34A12A2TMV NN 5 TMV NB 5 TMV 94%~99%CZ+2A2 TMV 50 74.9 S572.08 1007-5119201303-0110-03 DOI 10.3969/j.issn.1007-5119.2013.03.22 Disinfection Effect of Antagonistic Bacteria Cultures on Tobacco Mosaic Virus * SHEN Lili, YANG Jinguang, QIAN Yumei, ZHAI Xilun, WANG Fenglong （Key Laboratory of Tobacco Pest Monitoring Controlling Integrated Management, State Tobacco Monopoly Bureau, Tobacco Research Institute of CAAS, Qingdao 266101) Abstract: Tobacco mosaic virus (TMV) is one of the major diseases on tobacco, spreading fast and popular early. Mechanical contact infection in farm work and transmission of virus residues in soils are the major culprits for the rampant outbreak of TMV. For application of bio-control bacteria in soil and instruments disinfection, the suppression in vitro of the five single bacteria cultures and the twain bacteria cultures on TMV was investigated on local lesion host of Nicotiana tabacum var. samsum NN challenged by mechanical inoculation of mixture of cultures with TMV. The results showed that both of the five single bacteria cultures and the twain bacteria cultures had good disinfection effect on TMV, all of 5-fold dilution cultures suppressed TMV from 94% to 99% in vitro compared to the mixture of NB with TMV. And the twain bacteria cultures of CZ and 2A2 had the best disinfection effect, with 74.90% of suppression in vitro by 50-fold dilution cultures. These results indicated that CZ and 2A2 has a potential to be an instruments and soils disinfector against TMV. Keywords: antagonistic bacteria; cultures; tobacc