非水相生物催化制备甘油磷酸胆碱 - 食品科学.pdf

※工艺技术 食品科学 2012, Vol.33, No.22 67 * 毛慧芳，张洪斌 ，赵艳艳，胡雪芹 (合肥工业大学医学工程学院，安徽 合肥 230009) 摘 要：通过在非水相中对生物催化剂脂肪酶水解大豆卵磷脂生成甘油磷酸胆碱的研究，确定最佳的工艺条件。结 果表明：最佳条件为反应时间6h、反应温度40 ℃、pH5.0 、加酶量12.5mg/mL、水含量4% ，为了增加酶的利用率和 减少有机溶剂的使用，反应1.5h后补加底物，补加质量为初始质量的50%，补加的底物质量浓度为0.437g/mL 。在此 工艺条件下，经高效液相色谱检测分析，3次平行实验测得反应液经水萃取后甘油磷酸胆碱产率为97% 。该工艺为 甘油磷酸胆碱的制备提供了一种可行的方法。 ：脂肪酶；卵磷脂；甘油磷酸胆碱；非水相 Biocatalytic Production of Glycerophosphatide Choline in Non-aqueous Phase * MAO Hui-fang ，ZHANG Hong-bin ，ZHAO Yan-yan ，HU Xue-qin (School of Medical Engineering, Hefei University of Technology, Hefei 230009, China) Abstracts ：This study was undertaken to optimize reaction conditions for the biocatalytic deacylation of phosphatidylcholine (PC) by lipase to glycerol-3-phosphocholine (GPC) in non-aqueous phase. The optimal reaction conditions were found to be reaction at 40 ℃ and pH 5.0 for 6 h with an enzyme dose of 12.5 mg/mL in the presence of 4% water. Moreover, in order to increase the utilization rate of lipase and reduce solvent consumption, additional PC at a concentration of 0.437 g/mL was added in a proportion of 50% relative to the initial amount after 1.5 h during the reaction process. Under these process conditions, the average yield of GPC after aqueous extraction of reaction products was 97% (n = 3) as determined by HPLC. Thus, a feasible method to prepare GPC has been established. Key words ：lipase ；phosphatidylcholine ；glycerophosphatide choline ；non-aqueous phase 中图分类号： TS218 文献标识码：A 文章编号：1002-6630(2012)22-0067-05 大豆卵磷脂是大豆油生产的副产品，也是一种天 之外还表现出其他酶的活性，如磷脂酶、溶血磷脂