尿激酶型纤溶酶原激活物（upa）及其抑制物-1（pai-1）在.doc

尿激酶型纤溶酶原激活物（uPA）及其抑制物-1（PAI-1）在 子宫内膜癌中的表达及其临床病理意义 张玉阳，叶文蔚，韩义香，郑飞云 (温州医学院第一附属医院 妇产科，浙江 温州 325000) 【摘要】目的 探讨尿激酶型纤溶酶原激活物（uPA）及其抑制物-1（PAI-1）在子宫内膜癌发生、发展中的作用。方法 收集温州医学院附属第一医院子宫内膜癌标本61例，同时取患者相应的癌旁正常子宫内膜组织作为对照。应用组织芯片法与免疫组织化学法，检测每例子宫内膜癌标本和正常子宫内膜组织中uPA及其抑制物PAI-1的表达情况。结果 PAI-1在子宫内膜癌组织中的表达率为37.7％(23/61)，在正常子宫内膜组织中的表达率为60.7％(37/61)，两组间差异有统计学意义（χ2＝6.428，P＜0.05)；PAI-1的表达与患者的年龄、组织类型、肿瘤组织学分级、淋巴结转移无关(均P＞0.05)，但与临床病理分期有关(P＜0.05)。uPA在子宫内膜癌组织中的阳性表达率为42.6％(26/61)，在正常子宫内膜组织中的阳性表达率为21.3％(13/61)，两组间差异有统计学意义(χ2＝6.369，P＜0.05)；uPA的表达与患者的年龄、组织类型、肿瘤组织学分级无关(均P＞0.05)，但与肿瘤临床病理分期和肿瘤淋巴结转移有关(P＜0.05)。此外，uPA和PAI-1在子宫内膜癌中阳性表达差异无统计学意义(P＞0.05)。结论 uPA和PAI-1的异常表达在子宫内膜癌的发生中有重要作用，PAI-1的低表达和uPA的高表达与子宫内膜癌临床病理进展有关。 【关键词】子宫内膜癌；uPA；PAI-1；组织芯片；免疫组织化学 [中图分类号]：R737.310.5 Clinicopathological significance of expressions of uPA and PAI-1 in carcinoma of endometrium ZHANG Yu-yang，，，ENG Fei-yun. Department of Obstetrics and Gynecology, the First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000 【Abstract】Objective To investigate the role and clinicopathological significance of and uPA expressions in carcinoma of endometrium。Methods We collected samples of carcinoma of endometrium tissue and 61 samples of corresponding normal endometrium tissues in the First Affiliated Hospital of Wenzhou Medical College．The expression of and uPA was detected with tissue array and immunohistochemistry．Chisquare test and Spearman Correlation analysis were used to analyze the results．Results The positive rate of protein expression in cancer tissues was 37.7％(23/61)，higher than in nomal esophageal epithelia 60.7％(37/61)，with a significant differenceχ2＝6.428，P＜0.05)． difference was not found in uPA expression among patients with different degree of differentiation and different depth of invasion．expression levels of uPA in carcinoma of endometrium tissue and normal endometrium tissues were 42.6％(26/61) and 21.3％(13/61)，with a significant difference(χ26.369，P＜0.05)．he expression level of PAI-1 was not related to the degree of diffe