单步种子溶胀法与二步种子溶胀法制备氯霉素分子印迹聚合物微球的 .pdf

化 工 进 展 2011 年第 30 卷第 2 期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·381 · 研究开发 单步种子溶胀法与二步种子溶胀法制备氯霉素分子印迹 聚合物微球的比较 左华敏，李 雁，李 璐，解新安，熊明州 （华南农业大学食品学院，广东广州 510642 ） 摘 要：以聚苯乙烯乳液为种球、氯霉素为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联 剂，采用单步种子溶胀法与二步种子溶胀法制备了单分散氯霉素分子印迹聚合物微球，用光学显微镜和扫描电 镜对微球的形貌进行了表征，并用静态吸附实验考察了微球的吸附识别性能。结果表明，二步种子溶胀法制备 的微球分散性、吸附识别性能最佳，平均粒径为8.44 µm，分散系数为14.2%，分离因子为3.39。 关键词：单步种子溶胀；二步种子溶胀；氯霉素；分子印迹 中图分类号：O 636.1 文献标志码：A 文章编号：1000 –6613 （2011 ）02 –0381 –05 Comparison of preparation of molecularly imprinted polymer microspheres for chloramphenicol by one-step seed swelling and two-step seed swelling ZUO Huamin ，LI Yan ，LI Lu ，XIE Xin ’an ，XIONG Mingzhou （College of Food Science，South China Agricultural University，Guangzhou 510642，Guangdong，China ） Abstract：The monodisperse molecularly imprinted polymer microsphere（MIPMs ）for chloramphenicol was prepared by one-step seed swelling and two-step seed swelling method using linear polystyrene emulsion as seeds ，methacrylic acid （MAA ）as functional monomer and ethylene glycol dimethacrylate （EDMA ）as crosslinker ，respectively. The surface features of MIPMs were analyzed with optical microscope and SEM ，and the adsorption properties of microspheres were analyzed with static adsorption experiments. The results showed that dispersion coefficient ，separation factor of microspheres made by two-step seed swelling were better ，the average size was 8.44 µm ，dispersion coefficient was 14.2% ，separation factor was 3.39. Key words：one-step seed swelling；two-step seed swelling ；chloramphenicol；molecularly imprinted 分子印迹技术以目标分子为模板分子，将具有 危害[2] 。近年来，国内外发展了一些氯霉素残留分 结构上互补的功能化聚合