不同玻璃化冷冻载体对人卵巢组织冷冻效果的影响 - 蚌埠医学院学报.pdf

蚌埠医学院学报2014年12 月第39卷第12期 1611 [文章编号] 1000鄄2200(2014)12鄄1611鄄04 ·基础医学· 不同玻璃化冷冻载体对人卵巢组织冷冻效果的影响 1 2 郑摇 轶 ,周摇 平 [摘要]目的:比较不同的玻璃化冷冻载体对人卵巢组织冷冻效果的影响,为人卵巢组织玻璃化冷冻方案的选择提供理论基础 和实验参考依据。 方法:预处理人卵巢皮质切成 10 mm伊1 mm伊1 mm大小,随机分为新鲜组(A组)、针浸润玻璃化冷冻组 (B组)、表面玻璃化冷冻组(C组)和直接覆盖玻璃化冷冻组(D组)。 A组直接予以固定、组织切片及HE染色;B、C、D3组采 用不同玻璃化冷冻载体冷冻后,投入液氮保存2个月,采用快速复温法解冻后予以固定、切片及染色。 行形态学分析,通过计 算各组卵巢组织切片中正常形态始基卵泡率,比较各组冷冻效果。 结果:4组共计数809个始基卵泡,其中正常形态始基卵泡 率分别为:A组96.12%,B组88.14%,C组80.00%,D组81.25%。 行玻璃化冷冻的3组卵巢组织正常形态始基卵泡率均明 显小于A组(P0.01);而B组、C组和D组正常形态始基卵泡率差异均无统计学意义(P0.05)。 结论:玻璃化冷冻后人卵 巢组织正常形态始基卵泡率较新鲜组织下降,但仍能较好地保存人类卵巢组织,可用于人类卵巢组织的冷冻保存。 [关键词] 卵巢组织;玻璃化冷冻;始基卵泡 [中国图书资料分类法分类号] R322.65摇 摇 摇 [文献标志码]A Effect of different vitrification carriers on the freezing human ovarian tissue 1 2 ZHENGYi ,ZHOU Ping (1.Department of Gynecology and Obstetrics,The FirstAffiliated Hospital of BengbuMedical College,BengBuAnhui233004; 2.Reproductive Medicine Center,The FirstAffiliated Hospital of AnhuiMedical University,HefeiAnhui230031,China) [Abstract] Objective:To compare the effects of different vitrification carriers on the freezing human ovarian tissue,and provide the theoretical and experimental basis in selecting frozen solution.Methods:The human ovarian cortex tissues were cut into the size of 10 mm伊1mm伊1mm,randomly divided into thefresh group(group A),needle immersed vitrification group(group B),solid surface vitrification group(group C) anddirectcoveredvitrificationgroup(groupD)accordingtodifferentvitrificationcarriers.Thefreshgroup werefixed by 10% formaldehyde,embeded in paraffin,sectioned and stained with hematoxylin and eosin.The other three groups were pr