赭曲霉毒素a 核酸适配体筛选 - 食品安全质量检测学报.pdf

6 9 Vol. 6 No. 9 2015 9 Journal of Food Safety and Quality Sep. , 2015 A 1, 2, 3 4 1, 2, 3 1, 2, 3 1, 2, 3* 刘继红 , 胡 颖 , 洪慧杰 , 张 玲 , 王红旗 ( 1. , 450002; 2. , 450002; 3. (), 450002; 4. , 450002) 摘 要: 目的 A 方法 A 结果 A , ; , A BN- 结论 SELEX A , 关键词: ; ; ; ; Screening of DNA aptamer of ochratoxin A 1, 2, 3 4 1, 2, 3 1, 2, 3 1, 2, 3* LIU Ji-Hong , HU Ying , HONG Hui-Jie , ZHANG Ling , WANG Hong-Qi (1. Institute of Quality Standard and Testing Technology for Agro-products, Henan Academy of Agricultural Science, Zhengzhou 450002, China; 2. Henan Key Laboratory of Grain Quality and Safety and Testing, Zhengzhou 450002, China; 3. Laboratory of Quality Safety Risk Assessment for Agro-Products (Zhengzhou), Ministry of Agriculture , Zhengzhou 450002, China; 4. Henan Agricultural University , Zhengzhou 450002, China) ABSTRACT: Objective To screen aptamer of ochratoxin A (OTA) in wheat and other related food. Methods The solid-target selection strategy and magnetic-separation selection method were adopted to screen aptamer of ochratoxin A. Results The OTA aptamer had high affinity with nanomolar dissociation constants and