微生物浊度法与管碟法测定乙酰吉他霉素颗粒剂的含量比较.pdf

2010 11 1 56 Drug Standards of China 2010, Vol11No1 高燕霞, 姜建国, 韩彬(, 050011) :建立浊度法测定乙酰吉他霉素颗粒剂的方法, 并与管碟法进行 较: 浊度法以金黄色葡萄球菌为试验菌, - 1 加菌量 20% 25% (V /V), ( 37 05) 培养 4 5 hr左右测定: 浊度法抗生素线性浓度为 045 63 IU mL 与 管碟法测定含量 较, 无显著性差异: 浊度法灵敏, 快速, 影响因素较少, 与管碟法均可作为测定乙酰吉他霉素颗粒剂 效价的方法并存 :效价; 浊度法; 管碟法; 乙酰吉他霉素颗粒剂 : 9212 : A : 1009- 3656(2010) - 1- 56- 3 TheComparision of the ContentDetermination of Acetylkitasamycin Granules by Turbid-i metricMethod and AgarDiffusionMethod Gao Yan-xia, Jiang Jian-guo, H an Bin (H ebei I nstitu tef or D rug Con trol, Sh ij iazhuang 050011) Abstract Objective: To estab lish a turb id mi etric method for the content determ ination of Acetylk itasamycin G ranu les, com paredw ith agar d iffusion methodM ethod: The test organism w as Staphylococcus aureus and cu lturedw ith fresh m ed ium ( pH 70) Adjust spore suspension of Staphylococcus aureus inoculatew ith a 20% 25% (V /V) ratio at ( 37 05) for about 4 5 h rResults: The lin- ear range of Acetylk itasamycin was 045 63 IU mL- 1 And the contents of two m ethods had no sign ificant difference Conclusion: The turbid mi etricm ethodw as sensitive, rapid and feasible to determ ineA cetylkitasamycin Granules, it can concom itance w ith agar d iffusion m ethod eyW ords: Potency; turb id mi etricm ethod; agar diffusion m ethod; Acetylk itasamycin Granules 16 , , 1953( Streptomyces , , K itasatoensis) , , , , , 1 , CBZ- 1 , , , ( 1 cm) ()CAM - A , ( ) (Stap hylococcus aureus) [ CMCC , ( B) 26 003], ; [ 1] ( 130372-200301, 668