金银花多酚类物质的纯化研究 - 天津中草药杂志社.pdf

Drug Evaluation Research 第 35 卷 第 3 期 2012 年 6 月 - 185 - 金银花多酚类物质的纯化研究 * 陈 奇，邓雁如 ，夏 娜 天津中医药大学，天津 300193 摘 要：目的 考察常用的大孔吸附树脂对金银花多酚类物质的纯化效果，筛选纯化工艺条件。方法 以总酚的质量分数和 回收率作为指标，采用静态吸附及动态吸附考察树脂的吸附、解吸性能。结果 NKA-2 型树脂对多酚类物质的吸附能力明 显强于其他树脂，其载药量为 7 倍树脂体积，水洗 5 倍树脂体积，60%乙醇洗 6 倍树脂体积。此条件下，浸膏量减少 40%左 右，总酚质量分数提升近一倍，达到 60%左右，样品回收率达到 55% 。结论 NKA-2 型树脂对金银花多酚类的纯化效果较 好，适用于金银花多酚类物质的分离纯化。 关键词：多酚；有机酸；黄酮；纯化；NKA-2 中图分类号：R284.2 文献标志码：A 文章编号：1674 - 6376 (2012) 03 - 0185 - 05 Purification of polyphenols from Lonicerae Flos CHEN Qi, DENG Yan-ru, XIA Na Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China Abstract: Objective To study the process parameters for purification of polyphenols from Lonicerae Flos by commonly used macroporous resins, and to optimize the purification technological conditions. Methods The purity and recovery of polyphenols were taken as indexes, and the adsorption and desorption performance by difference resins were studied by using static adsorption and dynamic adsorption. Results It has been found that NKA-2 resin’s adsorption capacity of polyphenols was the best among those resins. The optimum condition is seven volume times of sample, five volume times of water washing, and six volume times of 60% ethanol washing. Under those conditions, 40% extract was reduced with the purity of polyphenols around 60%, and the recovery rate was 55%. Conclusion NKA-2 resin has a good performance and is suitable for the production of polyphenols from Lonicerae Flos. Key words: Lonicerae Flos ; polyphenols; organic acids; flavonoids; purification; NKA-2 金银花为中医常用药，具有清热解毒、疏散风 NKA-2 、SP850、D101 及 AB-8 树脂的纯化效果， 热等功效[1] ，其有效成分主要为多酚类物质，包括 筛选出树脂分离纯化的最优工艺参数，为金银花多 绿原酸、异绿原酸等酚酸类物质及木犀草苷等黄酮 酚类的纯化提供参考。