固定化解脂耶氏酵母菌处理含油废水的工艺条件摸索 - 环境工程学报.docVIP

w拟再审修改稿200708050Yarrowia lipolytica） 处理油脂废水的性能研究 吴兰1,2, 万金保2* （1.南昌大学生命科学学院，南昌 330047；2. 南昌大学环境科学与工程学院、教育部鄱阳湖湖泊生态暨生物资源利用实验室， 南昌 330047） 摘 要 以海藻酸钠为固定化载体材料，以氯化钙作为交联剂，将高效降解油脂菌——解脂耶氏酵母（Yarrowia lipolytica）包埋制备成固定化微生物小球，用以处理油脂废水，考察了最佳处理条件。结果表明，解脂耶氏酵母经固定化包埋后处理色拉油废水的最适条件为：温度25～35℃、pH4～8、摇床转速100～200r/min，处理初始油浓度在2000mg/L，包埋菌浓度6.65×106个/mL，包埋量为4mL/150L。与悬浮状态相比，固定化微生物温度适应范围增大、热适应性增强、pH值往酸性方向偏移。将固定化解脂耶氏酵母投入YPD液体培养基内驯化30 h再处理色拉油废水，结果显示：驯化后的固定化微生物在同样条件下对色拉油的降解率达到82%，比未驯化状态高20%，且驯化后的机械强度、稳定性和重复使用性都有明显改善。 关键词 固定化、海藻酸钙、解脂耶氏酵母、油脂废水 中图分类号 文献标识码 A 文章编号 Investigation on the capability of disposing the grease wastewater with Immobilized Yarrowia lipolytica Wu Lan1,2 ， Wan Jinbao2* （1.The college of Life Science, Nanchang University, Nanchang 330047 2.The college of Environmental Science and Engineering, Nanchang University; Key Lab．of Poyang Lake Ecology and Bio-resource Utilization ，Nanchang University，Ministry of Education, Nanchang 330047） Abstract The optimal conditions have been studied in disposing the grease wastewater with predominant disposing oil bacteria——immobilized Yarrowia lipolytica embedded into microorganisms when using sodium alginate as immobilized carrier and calcium chloride as crosslinker. The results show that the optimal conditions of disposing salad oil for immobilized Yarrowia lipolytica are: temperature 25～30 ℃, pH 4～8, the shaking speed 100～200 r/min, primary oil concentration 2000 mg/L, embedded bacteria concentration 6.65×106 entries/mL, embedding quantity 4mL/150L. In contrast with suspended bacteria, the embedded microorganisms represent more adaptable capability of a larger range of temperature and an enhancement of heat resistance，a pH avertion to acidity. The result of plunging the immobilized Yarrowia lipolytica into YPD liquid substrate to domesticate for 30 h before disposing salad oil shows that: after domestication, disposing rate of salad oil for the immobilized microorganisms can be 82%, with an increase of 20% c