Techniques Optimization of Combined Enzymatic Hydrolysis on Brewers＇ Spent Grain from Novozymes.pdf

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Techniques Optimization of Combined Enzymatic Hydrolysis on Brewers＇ Spent Grain from Novozymes.pdf

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JournalofLifeSciences6(2012)1232—1236 TechniquesOptim izationofCombinedEnzymatic HydrolysisonBrewers’SpentGrainfrom Novozymes ZhaoxiaLi1JinlongYan，DanShen2andChengDing2 ，，_SchoolofChemicalandBiologicalEngineering,YanchengInstituteofTechnology,Yancheng224051，China 2SchoolofEnvironmentalScienceandEngineering,YanchengInstituteofTechnoloyg，Yancheng224051，China Received：August23，2012／Accepted：October09，2012／Published：November30 2012 ， Abstract：Purpose：Toextractprotein，decreasethecelluloseandfacilitatethedigestionandabsorptionofbrewers’spentgrainby anima1．Topic：DiscussandoptimizethehydrolysisconditionsofthecombinedenzymatichydrolysisbyNovozymes ． M ethod：The fresh brewers’spentgrainwasfirstly dried smashedand sifted．Thenasindicatorsoftheprotein extractionrateintheenzyme ， solutionandthecontentofcelluloseintheindex，theparametersofenzymatichydrolysis suchasthesolid．1iquidratio ． reaction temperature，pH，enzymedosageandreactiontime，wereinvestigatedindetailed．Afterhydrolysis，thebrewers’spentgrainwasput intheboilingwaterbathforinactivationofr15minutesandcentrifuged，thesupernatantswerevolumetol0OmL andtheprotein contentwasmeasured．Aftertheprecipitatewasdried，thecellulosecontentwasalso measured ． Achievements：Theoptimized conditionswerewithtemperatureof50。C，pH 6．5，enzymeamountof30mgofrNovozymesenzymeand2．5hforreactiontime ． Under