UPLC_MS_MS法测定血液中5种吩噻嗪类药物_赵蒙.pdfVIP

21 6 Vol. 21No. 6 70 2012 11 CHEMICAL ANALYSIS AND METERAGE Nov. 2012 100040 5 HSS SB C18 –0.1% – ESI+ MRM 62%65%71%97%66%107%72%95%77%96%0.020.010.010.010.01 ngmL n 7% =60.0520 ngmL0.999 Determination of 5 Phenothiazine Drugs in Human Blood by UPLC–MS–MS Zhao MengWang ZhaohongLiu YongLyu Jungang Procuratorial Technical Information Center of Supreme Peoples ProcuratorateBeijing100040China The method of determination of 5 phenothiazine drugs in human blood was established by super high performance liquid chromatography tandem mass spectrometry(UPLC–MS–MS). Blood sample was separated by the acetonitrile – 0.1% formic acid aqueous solution gradient elution in HSS SB C18 column after precipitated proteinand analyzed with electrospray ionization (ESI+) and reaction monitoring(MRM) way. The recoveries of promethazine chlorprothixenechlorpromazineperphenazinetirfluopazine were 62%–65%71%–97%66%–107%72%–95%77%– 96%, respectivelyand the detection limits were 0.020.010.010.010.01 ngmL, respectively. The relative standard n deviation of determination results was less than 7% ( =7). The linear range was 0.05–20 ngmLthe linear correlation coefficients of promethazinechlorprothixenechlorpromazineperphenazinetirfluopazine were more than 0.999. The method is suitable for determination of phenothiazine drugs in human plasma. super high performance liquid chromatography tandem mass spectrometryUPLC–MS–MS; phenothiazine drugs; human plasma 5 18 h 1.1 1 TQ–S