植物激素母液配制.doc

1-Naphthaleneacetic acid (NAA) 27/9申请，2007-10-15收到 上海稼丰园艺用品有限公司 Lot:070625 50 g 30元 货号Y2285 BR级、进口 纯度 99.5% C12H10O2 MW: 186.2 Store at RT Phytechnology Laboratories, LLC P.O.Box 13481 Shawnee Mission, KS 66282-3481 08-3-25配母液10 mL (1.0 mg/mL) 参照Agrobacterium protocols 2ed vol2 p27方法配制： α-Naphthalene acetic acid (NAA) (Duchefa): Made as 1 mg/mL stocks by dissolving 20 mg of NAA in 1 mL 1 N NaOH and making up to volume with 19 mL of distilled water. Stock solution can be stored at 4°C for 6 wk. 即称10 mg NAA，溶于0.5 mL 1N NaOH，加9.5 mL ddH2O，分装1 mL/管，-20℃保存。 2009-12-6配NAA母液10 mL (1.0 mg/mL) ----同前。 2,4-Dichlorophenoxyacetic acid (2,4-D) 20/6申请，2008-7-3收到 上海稼丰园艺用品有限公司 Lot:080210 100 g 50元 货 BR级、进口 纯度 99.8% C8H6O3Cl2 MW: 221.04 Store at RT Phytechnology Laboratories, LLC P.O.Box 13481 Shawnee Mission, KS 66282-3481 08-7-5配母液25 mL 2,4-Dichlorophenoxyacetic acid (2,4-D) (Sigma-Aldrich), 1 mg/ml in ethanol/water (dissolve powder in ethanol then add water to volume). Filter sterilise, and store at -20°C in 1 ml aliquots. (From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat, Huw D Jones et al. Plant Methods 2005, 1:5) 即称25 mg 2,4-D，溶于15 mL左右 ethanol，缓慢加5 mL MilliQ H2O，用乙醇定容25 mL，分装1 mL/管，-20℃保存。（注：有的说用1 N NaOH溶解，有的用少量乙醇溶解，为避免析出，故多加了很多乙醇。另：2,4-D可以高温高压灭菌—参见Duchefa） 09-10-15配2,4-D母液 Prior to use, check to be sure 2,4-D is dissolved. If crystals are present in the stock solution, warm to room temperature to dissolve. If the crystals do not dissolve, prepare a new stock solution. A working solution of 2,4-D (1.0 mg/mL) can be prepared by dissolving 100 mg in 70 mL of ethanol, adding 30 mL of distilled water, vortex-mixing, filter sterilizing, and storing 1 mL aliquots at –20°C.（Transgenic Plants Methods and Protocols，2004，pp199） (+)-cis,trans-Abscisic acid (S-脱落酸) 20/6申请，2008-7-3收到 上海稼丰园艺用品有限公司