大鼠焦虑模型血浆神经肽Y的变化.pdfVIP

JournaI of MedicaI Forum Vol。29 No。5 March 2008 · 5· 大鼠焦虑模型血浆神经肽Y的变化 王金宝，朱秀娥，刘纪猛，谢 正，李恒芬 新乡医学院第二附属医院 新乡市 453002 摘要 目的 探讨大鼠的焦虑状态血浆神经肽Y的变化。方法 ①将l6只sD大鼠按体重随机分为两 组：对照组和模型组。采用空瓶刺激法制造焦虑模型。②放射免疫分析法(RIA)检测大鼠血浆神经肽Y浓度。 ③统计分析分别采用单因素方差分析和独立t检验。结果 ①空瓶应激行为学结果发现，造模组的攻击行为和 修饰行为得分均高于对照组，差异有显著性意义(P0．05)。②模型组大鼠血浆NPY浓度明显高于对照组 (P0．05)。结论 焦虑状态下大鼠血浆神经肽Y水平增高。 关键词 焦虑症；神经肽Y；大鼠模型 中图分类号：R一332 文献标识码：A 文章编号：1672-3422(2008)05-0005-03 Variation of Serum Neuropeptide Y in Anxiety Model Rats WANG Jinbao，ZHU Xiue，LIU Jimeng，et al The Second Affiliated Hospital ofXinxiang Medical College，XXnxiang 453002，China ABSTRACT Objective To investigate the change of serum neuropeptide Y in anxiety model rats．Methods ①Total 16 rats were randomly divided into 2 groups by weight：control group and model group．Apply vacant bottle stimulus method to produce anxiety mode1．②The rat blood plasma NPY concentraion was detected by radio immunoassay(RIA)．③Statistical analysis was correspond— ently used Oneway ANOVA and independent t test．Results(~)Vacant bottle stimulate Ethology result： scores of the model group including aggressive behaviour，modify behavior was higher than that of the blank control group，The difference has significant meaning(P0．05)．②The blood plasma neu— ropeptide Y concentration of model group was much higher than that of the group(P0．05)。Con· clusion Rats serum levels of neuropeptide Y rised at anxiety status． KEY WORDS Anxiety；Neuropeptide Y；Rat Model 焦虑症作为一种复杂的情绪障碍疾病，其发 明，除应激期间外，动物自由饮水和摄食。②主要 病是由心理、社会、生理等多方因素共同作用的结 仪器：西安二六二厂产FJ一2008P型 一计数仪。 果…。虽然医学界已就其发病机制进行了大量的 ③主要试剂：大鼠神经肽Y(Neusopeptide Y，NPY) 研究，但目前尚未有准确的生物机制理论。NPY 放免试剂盒购自解放军总医院科技开发中心放免 自从1982年被Tatemoto 从猪脑组织中纯化以 所。药盒组成：缓冲液 1瓶；NPY血清 1瓶； 来，逐渐成为医学界的研究热点，