中国明对虾卵黄蛋白原部分cdna 序列和表达部位研究 - 河北工业科技.pdf

27 1 Vol. 27, No . 1 2006 3 Journal of Hebei University of Science and T ec nology Mar . 2006 : 1008-1542( 2006) 01-0049- 05 cDNA 孙丽娜, 柳峰松, 谢 松 ( 河北大学生命科学学院, 河北保定 071002) : 从成熟的中国明对虾卵巢中提取总RNA, 经同源克隆得到 卵黄蛋白原( Vg) 部分 cDNA 序列, 长度为1 226 bp在NCBI 上比对后发现它与墨吉明对虾短沟对虾等的Vg mRNA 序列有 很高的相似性根据8 种虾( 墨吉明对虾短沟对虾凡纳滨对虾日本囊对虾刀额新对虾罗氏沼 虾高背长额虾和中国明对虾) Vg mRNA 相应序列建立 系统发生树通过 RT-PCR 证实 雌 虾的卵巢和肝胰腺是卵黄蛋白原的合成位点 : 中国明对虾; 卵黄蛋白原; cDNA; 同源克隆; 表达部位 : Q786 : A Cloning of partial cDNA of vitellogenin from Fennerp op enaeus Chinensis and study of its expressive site SU N L-i na, LIU Feng-song, XIE Song ( College of Life Science, H ebei Universit y, Baoding H ebei 071002, C ina) Abstract: Partial cDNA of vitellogenin, 1 226 bp, w as cloned t roug omologous cloning met od from t e overy of mature s rimp, Fennerop enaeus Chinensis . T ere are ig identities in vitellogenin mRNAs between t is sequence of Fennerp op enaeus and M erguiensis ( AY499620) and P enaeus semis ulcatus (AY051318) . A p ylogenetic tree w as establis ed based on t e corre- sponding vitellogenin mRNA of eig t species, including Fennerp op enaeus, Chinensis, Fennerp op enaeus, M erg uiens is, Penaeus, Semis ulcatus, L itop enaeus Vannamei, M ars up enaeus J ap onicus, M etap enaeus Ensis, M acrobrachi um Rosenbergii and Panda- lus H p sinotus . It w as proved t at vitellogenin is synt esized in ovary and epat opancreas in mature female s rimp t roug t e RT-PCR met od. Key words: Fennerp op enaeus Chinensis; vitellogenin; cDNA; cloning; expressive site , ( Vitellin, V t) , Vt , Vt [ 1] ( Vitellogenin, Vg) , Vt V g , , Vt Vg