水稻广亲和基因s5-n 的功能标记开发及其应用 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2009, 35(11): 2000−2007 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.02000 水稻广亲和基因S5-n 的功能标记开发及其应用 杨 杰 王 军 曹 卿 陈志德 仲维功* / , 210014 : S5-n , F , F S5-n, , , 1 1 S-5 136 bp , InDel S513602428DualrCPSLO17 S5-n, S5136 PCR 3 , S5-n 3037 02428 F2 , S5-nS5-i , 121 , 554 (O. sativa L.)27 (O. rufipogen Griff.)24 13 , PCR S5-n, Kasalath ; 20 02428 , S5-n 2 S5-n 64S : S5-n; ; ; ; Development and Application of a Functional Marker for Wide Compatibility Gene S5-n of Rice * YANG Jie, WANG Jun, CAO Qing, CHEN Zhi-De, and ZHONG Wei-Gong Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice RD Center, Nanjing 210014, China Abstract: Wide compatibility varieties (WCVs) with S5-n are able to produce highly fertile hybrids when crossed to both indica and japonica varieties. The conventional breeding strategy of pyramiding S5-n is to test the fertility of F1 hybrid. However, this method is labour-consuming, time-consuming and difficult in operation. The technically simplified approach of marker-assisted selection(MAS) has directly and practically used in rice breeding. Therefore, it is urgent to find a convenient and efficient way in WCV breeding with MAS.