5 年生人参出苗期几种脱氢酶活力比较 - 中国现代应用药学.pdf

·专 栏· ·中药与天然药· 5 年生人参出苗期几种脱氢酶活力比较 刘宏，赵雨* ，邢楠楠，张惠，刘海龙(长春中医药大学研发中心，长春 130117) 摘要：目的 对 5 年生人参出苗期参根和参苗中苹果酸脱氢酶(malate dehydrogenase ，MDH) 、乳酸脱氢酶(lactate dehydrogenase ，LDH) 、乙醇脱氢酶(Alcohol dehydrogenase ，ADH) 、葡萄糖-6- 磷酸脱氢酶(glucose-6-phosphate dehydrogenase ，G6PDH)、α-磷酸甘油脱氢酶(α-glycerophosphate dehydrogenase ，α-GPD)5 种脱氢酶的活力进行比较。方 法 采用中性缓冲液提取粗酶液，应用分光光度法测定MDH ，LDH ，ADH ，G6PDH ，α-GPD 的活力。结果 在人参出 苗期，参根和参苗中的5 种脱氢酶活力变化趋势有所不同，并在不同时期出现峰值。结论 MDH ，LDH ，ADH ，G6PDH ， α-GPD 的活力可以作为人参生长过程中长势优劣的评价指标。 关键词：人参；脱氢酶；出苗期 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2012)02-0122-05 Comparison of Dehydrogenase Activity in 5-year Ginseng during Seedling Stage * LIU Hong, ZHAO Yu , XING Nannan, ZHANG Hui, LIU Hailong(Research and Development Center of Changchun University of Chinese Medicine, Changchun 130117, China) ABSTRACT: OBJECTIVE To compare the activities of malate dehydrogenase (MDH), lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), glucose-6-phosphate dehydrogenase (G6PDH) and α-Glycerophosphate dehydrogenase (α-GPD) in 5-year Ginseng Radix in seedling stage. METHODS Adopt neutral buffer solution to extract the coarse enzyme. Use spectrophotometry to test the activities of MDH, LDH, ADH, G6PDH, α-GPD. RESULTS In seedling stage of Ginseng Radix, there were different changes trends of these five dehydrogenase in root and sprout. And there were peak values in different stages. CONCLUSION The activities of MDH, LDH, ADH, G6PDH, α-GPD can be used as the evaluation indicators of quality of Ginseng Radix. KEY WORDS: Ginseng Radix; dehydrogenase; seedling stage 人参(Panax ginseng C. A. Meyer)是五加科多 在，并在植物的生长发育过程中起着十分重要的 年生草本植物人参的干燥根，是我国名贵中药材 作用。 之一。人参具有大补元气、复脉固脱、补脾益肺、 人参1年中的生长共分为6个物候期：出苗 生津安神的功效[1] 。研究表明人参中主要含有人参