五味子降糖有效部位的hplc 指纹图谱研究 - 中国现代应用药学.pdf

五味子降糖有效部位的HPLC 指纹图谱研究 章曙丹1,2 1 2 ，徐强 ，聂磊 (1. 山东大学药学院药物分析研究所，济南 250012 ；2.浙江省中药研究所，杭州 310023) 摘要：目的 对五味子降糖有效部位提取物的 HPLC 指纹图谱进行研究，以更有效地控制产品质量。方法 采用 HPLC 测定10 批样品并建立标准对照指纹图谱。色谱条件：Dikma-C18 色谱柱(250 mm×4.6 mm ，5 μm) ，流动相为甲醇-水，梯 度洗脱，流速l mL·min1 ，检测波长为 250 nm ，柱温为40 ℃。结果 建立了五味子降糖有效部位的 HPLC 对照指纹图 谱，标示出 9 个共有指纹峰。结论 HPLC 指纹图谱的建立为科学评价五味子降糖有效部位的质量提供了依据，有助于 提高质量控制水平。 关键词：五味子；有效部位；指纹图谱；高效液相色谱法 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2012)07-0617-04 Study on HPLC Fingerprints of the Antidiabetic-active Fraction of Schisandrae Chinensis Fructus 1,2 1 2 ZHANG Shudan , XU Qiang , NIE Lei (1.Research Institute of Pharmaceutical Analysis, School of Pharmacy, Shangdong University, Ji’nan 250012, China; 2.Research Center of Chinese Traditional Patent Medicine, Zhejiang Research Institute of Traditional Chinese Medicine, Hangzhou 310023, China) ABSTRACT: OBJECTIVE To study the HPLC fingerprints of the antidiabetic-active fraction of Schisandrae Chinensis Fructus for better quality control. METHODS Ten samples were analysed to establish the standard fingerprint of the effective parts of Schisandrae Chinensis Fructus by HPLC . The chromatographic conditions were: Dikma-C18(250 mm×4.6 mm, 5 μm) column at the temperature of 40 ℃ , a gradient mobile phase of methanol-water with a flow rate of 1 mL·min-1, the detection wavelength was set at 250 nm. RESULTS A standard HPLC fingerprint of the antidiabetic-active fraction of Schisandrae Chinensis Fructus was established with 9 indicated common peaks. CONCLUSION The established fingerprint is reliable and sensitive which could be used in the quality evaluation and control process of Schisandrae Chinensis Fructus products. KEY WORDS: Schisandrae chinensis Fructus ; active fraction; chromatographic fi