- 1、本文档共6页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
响应面法优化tnf-α抑制剂桥南霉素的固态发酵培养pdf
. 584 .
1001-8689(2015)08-0584-06
TNF-α
*
( 361102)
TNF-α HPLC
Plackett-Burman(P-B)
400g/L35g/L10.5g/L(NH ) SO 0.6g/L10LHPLC
4 2 4
4.3mg/L1.5mg/L186.7%
R978.1 A
DOI:10.13461/ki.cja.005585
Optimization of solid culture conditions for TNF-α inhibitor qiaonanmycin via
response surface methodology
Ma Yu-lei, Gao Jun, Hu Zhi-yu, Zheng Zhong-hui and Xu Qing-yan
(School of Life Science of Xiamen University, State-Province Joint Engineering Laboratory of Targeted Drugs
from Natural Products, Xiamen 361102)
Abstract Objective The solid fermentation culture conditions of compound qiaonanmycin were optimized
via response surface methodology. Methods Based on quantitative detection method of qiaonanmycin by HPLC, the
key fermentation factors were determined via P-B test, then their optimized concentrations were confi rmed through
response face method. Results The optimum solid fermentation culture medium compositions of qiaonanmycin
were: potato 400g/L, dextrose 35g/L, peptone 10.5g/L, and (NH ) SO 0.6g/L. Under this condition, the yield of
4 2 4
qiaonanmycin was 4.3mg/L, resulting in a 186.7% increase comparing with original production yield, and the
optimized yield was consistent well with theoretical value predicted by response face method. Conclusion The
optimized solid fermentation culture conditions were reliable and repeatable, it was warranted for large-scale
fermentation process.
Key words Solid fermentation; Response surface method; Qiaonanmycin
文档评论(0)