响应面法优化tnf-α抑制剂桥南霉素的固态发酵培养pdf.pdf

. 584 . 1001-8689(2015)08-0584-06 TNF-α * ( 361102) TNF-α HPLC Plackett-Burman(P-B) 400g/L35g/L10.5g/L(NH ) SO 0.6g/L10LHPLC 4 2 4 4.3mg/L1.5mg/L186.7% R978.1 A DOI:10.13461/ki.cja.005585 Optimization of solid culture conditions for TNF-α inhibitor qiaonanmycin via response surface methodology Ma Yu-lei, Gao Jun, Hu Zhi-yu, Zheng Zhong-hui and Xu Qing-yan (School of Life Science of Xiamen University, State-Province Joint Engineering Laboratory of Targeted Drugs from Natural Products, Xiamen 361102) Abstract Objective The solid fermentation culture conditions of compound qiaonanmycin were optimized via response surface methodology. Methods Based on quantitative detection method of qiaonanmycin by HPLC, the key fermentation factors were determined via P-B test, then their optimized concentrations were confi rmed through response face method. Results The optimum solid fermentation culture medium compositions of qiaonanmycin were: potato 400g/L, dextrose 35g/L, peptone 10.5g/L, and (NH ) SO 0.6g/L. Under this condition, the yield of 4 2 4 qiaonanmycin was 4.3mg/L, resulting in a 186.7% increase comparing with original production yield, and the optimized yield was consistent well with theoretical value predicted by response face method. Conclusion The optimized solid fermentation culture conditions were reliable and repeatable, it was warranted for large-scale fermentation process. Key words Solid fermentation; Response surface method; Qiaonanmycin