苏云金芽孢杆菌晶体蛋白分离纯化新方法研究 - 河北工业科技.pdf

28 4 河 北 工 业 科 技 Vol. 28, No . 4 2011 7 H ebei Journal of Industrial Science and T echnolog Jul 20 11 : 2011) ( 邢台广播电视大学理工系, 河北邢台 054000) : 分离苏云金芽孢杆菌( Bt) 晶体蛋白常用 方法有液体双相法密度梯度法沉淀法生物 物理分离法等, 但这几种方法各有优缺点从分离成本仪器设备纯度产量活性等方面考虑, 在 超声波处理悬液制备和紫外线辐射3 个方面进行了改进, 提出了一种新 分离Bt 晶体蛋白 方 法, 产品纯度可达90% 以上, 产率从原来 20% 提高到28% 本方法成本低, 产率高, 操作简单, 具有很强 实用价值 : 苏云金芽孢杆菌; 分离纯化; 晶体蛋白 : Q939 : A Isolation and purification of protein cr stal from Bt YANG Kaijie ( Pol technic Department, Xingtai Universit of Broadcast and T elevision, X ingtai Hebei 054000, China) Abstract: There are man methods such as liquid double phase, densit gradient, sediment, bioph sics separation and so on, frequentl used to isolate and purif the protein cr st al f rom bacillus thuringiensis ( Bt ) . H ow ever, these methods have their ow n defects as w ell as advantages. In this paper , the isolation cost, the equipment , the purit , the output and the activit were considered. T hree improvements on ultrasonic w ave process, suspension liquid preparation and ultraviolet ra radiation were made. T hus, a new met hod to separat e cr stal prot ein from Bt was set up. This technolog has a characteristic of low cost, simple operation and high application. The production rat io is improved from 20% to 28% w ith the product purit of 90% . Key words: bacillus thuringiensis( Bt) ; isolation and purification; protein cr stal ( bacillus thuringiensis, Bt) , , , , , , Bt , , , , , , , [ 1] [ 3] 1, 10% ~ 20% , , Bt , , Bt , Bt [ 2] , , 1 , 1. 1 : : :