抗蛇毒免疫球蛋白药物研发的现状和策略the current - 药学学报.pdfVIP

药学学报 Acta Pharmaceutica Sinica 20 15, 50 ( 12): 1573 1580 ·1573 · 蛇毒免疫球蛋白药物研发的现状和策略 靖, 范泉水, 郑 颖* (成都军区疾病预防控制中心, 云南 昆明 650032) 摘要: 抗蛇毒免疫球蛋白被公认为是目前治疗毒蛇咬伤最有效的 物。全世界绝大多数抗蛇毒免疫球蛋白来 源的主要途径是高免马匹血浆中多克隆抗体的分离。本文就抗蛇毒免疫球蛋白 物研发的历史和现状进行归纳, 并针对此类 物在生产及研发过程中的关键环 , 如免疫球蛋白lgG 及其片段分子特性的考虑, 免疫原的选择和 制备、免疫程序优化和蛋白分离纯化等方面进行简要综述, 以期为抗蛇毒 物的研发和生产提供参考和借鉴。 关键词: 蛇毒; 免疫球蛋白; 免疫血清; 免疫程序; 分离; 纯化 中图分类号: R943 文献标识码: A 文章编号: 0513-4870 (20 15) 12- 1573-08 The current situation and strategies of snake antivenomimmunoglobulins research and development * TIAN Jing, FAN Quan-shui, ZHENG Ying (Chengdu Military Region s Center for Disease Control and Prevention, Kunming 650032, China ) Abstract : Snake antivenomimmunoglobulins are considered to be the most efficient drugs in snake envenomings. Most snake antivenomimmunoglobulins all over the world are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum till now. In this review, we retrospect the history of snake antivenomimmunoglobulins, analysis the present situation and pay the close attention on the key technological links in the process of research and manufacturing, such as properties of lgG and its fragments, selection and preparation of immunogen, optimization of immunization schedule and proteinisolation and purification, which can be available for the reference in the research and development of snake antivenom. Key words: snake venom; immunoglobulin; immune serum; immunization schedule; isolation; pu