星点设计效应面法优化参归中风康复颗粒提取工艺范开苒.pdf

第 1 7 卷 第 9 期 辽宁中医药大学学报 Vol. 17 No. 9 2015 年 9 月 JOURNAL OF LIAONING UNIVERSITY OF TCM Sep .，2015 DOI：10.13194/j.issn.1673-842x.2015.09.018 星点设计 - 效应面法优化参归中风康复颗粒提取工艺 范开苒，姜英，程岚，王娇，康廷国 （辽宁中医药大学，辽宁 大连 116600 ） 目的：采用星点设计-效应面法参归中风康复颗粒的提取工艺进行优化。方法：以水煎法提取参归中 摘 要： 风康复颗粒中的有效部位，以出膏率和党参炔苷的含量为指标，采用星点设计-效应面法考察提取次数、溶媒用量、 提取时间3个因素对出膏率和党参炔苷的含量影响，采用综合评分法确定最佳提取工艺条件。结果：最佳提取工艺 为加水提取2次，溶媒量为9.5倍量，每次1.4 h。结论：星点设计-效应面法所建立的参归中风康复颗粒提取工艺的 数学模型预测性良好。 关键词：星点设计；效应面法；出膏率；党参炔苷 ( ) 中图分类号：R284.2 文献标志码：A 文章编号：1673-842X 2015 09- 0053- 03 Optimized Extraction of Shengui Stroke Rehabilitation Granule by a Centralcomposite Cesign/Response Surface Method FAN Kairan，JIANG Ying，CHENG Lan，WANG Jiao，KANG Tingguo （Liaoning University of Traditional Chinese Medicine，Dalian 116600，Liaoning，China） Abstract：Objective ：To optimize the extraction of Shengui Stroke Rehabilitation Granule by a central composite design/response surface method. Methods ：The effects of three independent variables，extraction times，ratio of material to solvent and extracting time were investigated with the content of lobetyolin and extracting rate. Response surfaces were delineated according to the mathematic models，and optimum formulations were selected. Results ：All the investigated response variables were found to be highly dependent on the formulation variables. The optimized values of extraction times，ratio of material to solvent