牦牛肺脏微血管铸型扫描电镜样品的制备方法.pdfVIP

2008年4月 甘 肃 农 业 大 学 学 报 第 4 3卷 第2期28～3O JOURNAL OF GANSU AGRICULTURAL UNIVERSITY 双 月 刊 牦牛肺脏微血管铸型扫描电镜样品的制备方法 王 宇 ，刘 英 ，俞红贤。，杜晓霞 ，柳东阳 ，赵建红 ， 赵海涛 ，李仁阁 ，刘 (1．甘肃农业大学动物医学院．甘肃 兰州 730070；2．青海大学畜牧兽医学院．青海 西宁 810016) 摘要：牦牛肺脏体积较大．在制备供扫描电镜观察的样品时存在着肺脏采取困难．灌注阻力大和血管易堵塞等 一 系列问题．为了解决这些问题．试验从肺脏的采取、填充剂的选择、灌注的部位和铸型标本的处理等方面对牦牛 肺脏微血管铸型扫描电镜样品的制备方法进行了探索．制出了品质优良的牦牛肺微血管铸型标本，总结出了制备 牦牛肺微血管铸型的方法． 关键词：牦牛；肺脏；微血管铸型；扫描电镜 中图分类号：S 852．13 文献标识码：A 文章编号：1003—4315(2008)02—0028—03 A method for making the preparation of pulmonary microvascu1ature cast under the scanning electron microscope(SEM)in yak WANG Yu ，LIU Ying。，YU Hong-xian。，DU Xiao—xia ，LIU Dong—yang ，ZHAO Jian—hong ， ZHAO Hal—tao ，LI Ren—ge ，LIU Bin (1．College of Veterinery Medicine．Gansu Agricultural University，Lanzhou 730070．China 2．College of Agricultural and Animal Sciences．Qinghai Uinversity，Xining 810016，China) Abstract：The lung was very large in yak and difficult to makingPreparation for the microvasculature under the scanning electron microscope．It was difficult to take out the1ungS from the thorax and the perfu— sion resistance was very large and the blood vessels were easy to be blocked．In order to solve these prob— lems，consideringthe collection of the lung，the choice of the filler，the site of perfusion and the disposal of the specimens，the method for making the pulmonary microvascular cast under the SEM on yek was ex— plored in the experiment．The excellent vascular cast was obtained after the repeated experiments．The method of making preparations for the pulmonary vascular cast on yak was summarized Key words：yak；lung；microvascular cast；scanning electron microscope(SEM) 血管铸型扫描电镜观察的方法 ，主要是用于