2006气相层析质谱仪-瓦里安科技.pptVIP

氣相層析質譜儀GC/MS 美商亞洲瓦里安科技(股)公司 GC基本原理 MS基本原理 數據處理 定性 定量 MS Tuning 與 日常保養 應用 GC基本原理 GC 基本組件 Injector------Capillary Injector Column ------Capillary Detector------Mass Recorder------Computer GC 簡圖 Capillary GC Review Universal or Selective GC Detectors Universal Detectors Flame Ionization Detector(FID) for Hydrocarbon Compounds Thermal Conductivity Detector(TCD) Selective Detectors Electron Capture Detector(ECD) Pulsed Flame Photometric Detector(PFPD) Thermionic Specific Detector(TSD) Information From GC Qualitation Retention Time Same compound has the same R.T. under the same GC conditions. Quantitation Peak Area Peak Height MS基本原理 The Basic Principle of Mass Spectrometry The Basic Principle of Mass Spectrometry CH3-CH2-CH2-CH3 CH3-CH2-CH2-CH3 CH3-CH2-CH2-CH3 Mass Spectrometry Inlet Systems GC, LC, Direct Probe Inlet….. Ion Source EI, CI…… Mass Analyzer Magnetic Sector, The Quadrupole Ion Trap….. Detector Electron Multiplier….. Ionization:Electron Impact Ionization:Chemical Ionization CH4 + e- → CH4+? + 2e- CH4+? + CH4 → CH3? + CH5+ CH5+ + M → CH4 + (MH)+ CH4 : CI gas M : Analyte Electron Impact Chemical Ionization Mass Analyzer:Magnetic Sector Mass Analyzer:External Source/Quadrupole Mass Analyzer:The Quadrupole Ion Trap Ion Detection:Electron Multiplier Sn/SnO or Pb/PbO doped glass funnel Shower of electrons is released when an ion strikes the wall The amplified ion current is sent to the electronic circuit and converted to a voltage signal 105~107gain Discrete Dynode Electron Multiplier Continuous Dynode Electron Multiplier GC Mass When MS Becomes a GC Detector GC/MS Can Provide More Information Retention Time Mass Spectra of Each Compound Library Search Compound Identification Structure Information Quantitation RTICC Quantitation Ion 數據處理 Data Process Qualitation Background Substract Identification of Coelution Peak Library Search Quantitation Build Calibration Curve Sample Analysis 定性 Reconstructed Total Ion Current Chroma